Simultaneous measurement of 19 components in serum-containing animal cell culture media by fourier transform near-infrared spectroscopy

Mark Riley, H. M. Crider, M. E. Nite, R. A. Garcia, J. Woo, R. M. Wegge

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Animal cell cultures generate maximal amounts of desired products when maintained in a controlled environment with low and constant concentrations of nutrients and wastes. Traditionally this has involved slow addition of glucose and glutamine; however, recent studies have indicated that a number of low concentration amino acids are required to prevent initiation of apoptosis. Therefore, optimal control of animal cell cultures will likely require measurement of a large number of chemical components. We present here the evaluation of a near-infrared spectroscopic (NIRS) monitoring scheme to quantify 19 cellular nutrients and wastes in culture medium with and without serum. The components include glucose, lactate, ammonia, pyruvate, glutamine, and 14 other amino acids. Spectroscopic calibrations were generated for a synthetic version of a standard culture medium (DMEM) in which the concentrations of 17 DMEM components and ammonia and lactate were varied in a random fashion. This randomization provides a stringent evaluation of the measurement scheme. Reasonably accurate measurements of these 19 components could be accomplished in the absence or presence of 10% horse serum by volume with percent errors ranging from 3% to 37%. Analytes with concentrations as low as 0.3 mM could be reliably quantified. The presence of serum, when properly included in the calibration, has little effect on measurement error. These results provide an important step toward application of NIRS for monitoring the large number of varying components of animal cell cultivations.

Original languageEnglish (US)
Pages (from-to)376-378
Number of pages3
JournalBiotechnology Progress
Volume17
Issue number2
DOIs
StatePublished - Jul 31 2001

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Near-Infrared Spectroscopy
Fourier transform infrared spectroscopy
near-infrared spectroscopy
Fourier Transform Infrared Spectroscopy
Culture Media
cell culture
Cell Culture Techniques
culture media
Glutamine
Ammonia
Calibration
Lactic Acid
Serum
glutamine
lactates
Amino Acids
Glucose
Controlled Environment
Food
animals

ASJC Scopus subject areas

  • Food Science
  • Biotechnology
  • Microbiology

Cite this

Simultaneous measurement of 19 components in serum-containing animal cell culture media by fourier transform near-infrared spectroscopy. / Riley, Mark; Crider, H. M.; Nite, M. E.; Garcia, R. A.; Woo, J.; Wegge, R. M.

In: Biotechnology Progress, Vol. 17, No. 2, 31.07.2001, p. 376-378.

Research output: Contribution to journalArticle

Riley, Mark ; Crider, H. M. ; Nite, M. E. ; Garcia, R. A. ; Woo, J. ; Wegge, R. M. / Simultaneous measurement of 19 components in serum-containing animal cell culture media by fourier transform near-infrared spectroscopy. In: Biotechnology Progress. 2001 ; Vol. 17, No. 2. pp. 376-378.
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