Simultaneous interphase cytogenetic analysis and fluorescence immunophenotyping of dedifferentiated chondrosarcoma: Implications for histopathogenesis

Julia A. Bridge, Joanne DeBoer, Joni Travis, Sonny L. Johansson, Göran Elmberger, Scott M. Noel, James R. Neff

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Cytogenetic analysis of four specimens (biopsy, definitive surgical, and two separately occurring lung metastases) of a dedifferentiated chondrosarcoma with a rhabdomyosarcomatous component revealed clonal karyotypic abnormalities in each. Anomalies seen in all specimens included a structurally aberrant chromosome 17 and extra copies of chromosomes 5, 7, 12, and 20. The derivation of the chromosomally abnormal cells was determined by a combined immunocytochemical/cytogenetic approach that allowed simultaneous assessment of cytogenetic aberrations and immunophenotypic features of individual cells. S-100 protein and desmin antibodies were used to evaluate the chondrosarcomatous and rhabdomyosarcomatous components, respectively. A chromosome 7-specific centromeric probe was used for determination of aneuploidy. In both specimens obtained from the primary lesion, S-100 protein and desmin-positive and-negative aneuploid cells were observed. These findings: 1) suggest that both the chondrocytic and rhabdomyoblastic cells arose from the same abnormal clone, 2) support the theory of a common primitive mesenchymal cell progenitor with the ability to differentiate or express features of more than one line of mesenchymal differentiation, and 3) indicate that the term dedifferentiated may be an inaccurate designation for this neoplasm.

Original languageEnglish (US)
Pages (from-to)215-220
Number of pages6
JournalAmerican Journal of Pathology
Volume144
Issue number2
StatePublished - Feb 1 1994

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Immunophenotyping
Chondrosarcoma
Cytogenetic Analysis
Interphase
Chromosomes, Human, Pair 7
Desmin
S100 Proteins
Fluorescence
Aneuploidy
Chromosomes, Human, Pair 5
Chromosomes, Human, Pair 17
Mesenchymal Stromal Cells
Cytogenetics
Chromosome Aberrations
Clone Cells
Neoplasm Metastasis
Biopsy
Lung
Antibodies
Neoplasms

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Bridge, J. A., DeBoer, J., Travis, J., Johansson, S. L., Elmberger, G., Noel, S. M., & Neff, J. R. (1994). Simultaneous interphase cytogenetic analysis and fluorescence immunophenotyping of dedifferentiated chondrosarcoma: Implications for histopathogenesis. American Journal of Pathology, 144(2), 215-220.

Simultaneous interphase cytogenetic analysis and fluorescence immunophenotyping of dedifferentiated chondrosarcoma : Implications for histopathogenesis. / Bridge, Julia A.; DeBoer, Joanne; Travis, Joni; Johansson, Sonny L.; Elmberger, Göran; Noel, Scott M.; Neff, James R.

In: American Journal of Pathology, Vol. 144, No. 2, 01.02.1994, p. 215-220.

Research output: Contribution to journalArticle

Bridge, JA, DeBoer, J, Travis, J, Johansson, SL, Elmberger, G, Noel, SM & Neff, JR 1994, 'Simultaneous interphase cytogenetic analysis and fluorescence immunophenotyping of dedifferentiated chondrosarcoma: Implications for histopathogenesis', American Journal of Pathology, vol. 144, no. 2, pp. 215-220.
Bridge, Julia A. ; DeBoer, Joanne ; Travis, Joni ; Johansson, Sonny L. ; Elmberger, Göran ; Noel, Scott M. ; Neff, James R. / Simultaneous interphase cytogenetic analysis and fluorescence immunophenotyping of dedifferentiated chondrosarcoma : Implications for histopathogenesis. In: American Journal of Pathology. 1994 ; Vol. 144, No. 2. pp. 215-220.
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