Simultaneous analysis of tumor and stromal gene expression profiles from xenograft models

Elizabeth Iorns, Jennifer Clarke, Toby Ward, Sonja Dean, Marc Lippman

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Identifying the gene expression alterations that occur in both the tumor and stroma is essential to understanding tumor biology. We have developed a dual-species microarray analysis method that allows the dissection of both tumor and stromal gene expression profiles from xenograft models, based on limited interspecies cross-hybridization on Illumina gene expression beadchips. This methodology allows for simultaneous genome-wide analysis of gene expression profiles of both tumor cells and the associated stromal tissue.

Original languageEnglish (US)
Pages (from-to)321-324
Number of pages4
JournalBreast Cancer Research and Treatment
Volume131
Issue number1
DOIs
StatePublished - Jan 1 2012

Fingerprint

Transcriptome
Heterografts
Neoplasms
Gene Expression
Microarray Analysis
Stromal Cells
Dissection
Genome

Keywords

  • Cross-hybridization
  • Dual-species
  • Gene expression profile
  • Microarray
  • Tumor microenvironment
  • Tumor stroma
  • Xenograft model

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Simultaneous analysis of tumor and stromal gene expression profiles from xenograft models. / Iorns, Elizabeth; Clarke, Jennifer; Ward, Toby; Dean, Sonja; Lippman, Marc.

In: Breast Cancer Research and Treatment, Vol. 131, No. 1, 01.01.2012, p. 321-324.

Research output: Contribution to journalArticle

Iorns, Elizabeth ; Clarke, Jennifer ; Ward, Toby ; Dean, Sonja ; Lippman, Marc. / Simultaneous analysis of tumor and stromal gene expression profiles from xenograft models. In: Breast Cancer Research and Treatment. 2012 ; Vol. 131, No. 1. pp. 321-324.
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