Simple and reliable genotyping protocol for mouse PrkdcSCID mutation

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Mutant mouse models, genetically-engineered or spontaneous-mutations, serve as valuable tools for biomedical research. Genotyping of mutant mice is a critical requirement for maintaining the colony, to breed with other mutants and to match the phenotypic observations. The SCID (Severe Combine Immuno Deficiency) mouse model has been extensively used as a common background-strain in many immunology and transplantation studies. Many different types of assays, including Restriction Fragment Length Polymorphism (RFLP), confronting two primer pairs PCR and end-point methods have been attempted for establishing a genotyping protocol for the SCID mutation. However, the best method that is thought to be reliable is sequencing, which requires additional time and resources to perform on a routine basis. In this report, we describe a novel RFLP assay that is simple and reliable. The method is validated by sequencing analysis, and this novel method can be adapted for routine genotyping of SCID model.

Original languageEnglish (US)
Pages (from-to)60-62
Number of pages3
JournalJournal of Immunological Methods
Volume431
DOIs
StatePublished - Apr 1 2016

Keywords

  • Genotyping
  • Humanized mice
  • NSG
  • RFLP
  • SCID

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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