Similarity of EPR Signal IIf rise and P-680+ decay kinetics in Tris-washed chloroplast Photosystem II preparations as a function of pH

Michael D Boska, Kenneth Sauer, William Buttner, Gerald T. Babcock

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

The rise time, of Signal IIf and the decay time of P-680+ have been measured kinetically as a function of pH by using EPR. The Photosystem II-enriched preparations which were used as samples were derived from spinach chloroplasts, and they evolved oxygen before Tris washing. The onset kinetics of Signal IIf are in agreement, within experimental error, with the fast component of the decay of an EPR signal attributable to P-680+. The signal IIf rise kinetics also show good agreement with published values of the pH dependence of the decay of P-680+ measured optically (Conjeaud, H. and Mathis, P. (1980) Biochim. Biophys. Acta 590, 353-359). These results are consistent with a model where the species Z (or D1) responsible for Signal IIf is the immediate electron donor to P-680+ in tris-washed Photosystem II fragments.

Original languageEnglish (US)
Pages (from-to)327-330
Number of pages4
JournalBBA - Bioenergetics
Volume722
Issue number2
DOIs
StatePublished - Feb 17 1983

Fingerprint

Photosystem II Protein Complex
Chloroplasts
Paramagnetic resonance
Kinetics
Spinacia oleracea
Washing
Electrons
Oxygen
P-680

Keywords

  • (Spinach chloroplast)
  • ESR
  • Electron transfer
  • P-680
  • Photosynthesis
  • Photosystem II

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

Cite this

Similarity of EPR Signal IIf rise and P-680+ decay kinetics in Tris-washed chloroplast Photosystem II preparations as a function of pH. / Boska, Michael D; Sauer, Kenneth; Buttner, William; Babcock, Gerald T.

In: BBA - Bioenergetics, Vol. 722, No. 2, 17.02.1983, p. 327-330.

Research output: Contribution to journalArticle

Boska, Michael D ; Sauer, Kenneth ; Buttner, William ; Babcock, Gerald T. / Similarity of EPR Signal IIf rise and P-680+ decay kinetics in Tris-washed chloroplast Photosystem II preparations as a function of pH. In: BBA - Bioenergetics. 1983 ; Vol. 722, No. 2. pp. 327-330.
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