Similarities and differences in the regulation of N-myc and c-myc genes in murine embryonal carcinoma cells

Thomas Sejersen, Magnus Rahm, Gabor Szabo, Sigurdur Ingvarsson, Janos Sümegi

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Abstract

c-myc and N-myc are closely related genes coding for putative DNA-binding proteins. The protein products of both genes have been implicated in the regulation of growth of normal and neoplastic cells. We compared the regulation of N-myc and c-myc expression under different growth conditions as well as in vitro differentiation of the murine EC lines F9 and PCC7. N-myc and c-myc expression was found to be regulated by distinct mechanisms, although similarities exist. Differences were found both at the transcriptional and at the post-transcriptional level. The two myc genes were regulated by mainly post-transcriptional mechanisms, but in PCC7 cells nuclear run-on assays indicated that c-myc was repressed at the level of transcription. N-myc and c-myc expression was negatively regulated at a post-transcriptional level in F9 and PCC7 cells during differentiation to visceral endoderm and nerve-like tissue, respectively. Serum stimulation of F9 cells for 4 h induced a sevenfold increase in c-myc transcripts but no significant elevation of N-myc transcripts. Mitogenic stimulation with insulin and transferrin also induced a marked elevation of c-myc but not of N-myc mRNA. In addition, the N-myc and c-myc genes differed in F9 cells with respect to (i) the kinetics of expression following induction of differentiation, c-myc undergoing quicker changes than N-myc; (ii) the response to cycloheximide inhibition of protein synthesis, indicating that c-myc but not N-myc is downregulated by a short-lived protein; and (iii) the half-lives of the transcripts, estimated to be approximately 40 min for c-myc and 130 min for N-myc.

Original languageEnglish (US)
Pages (from-to)304-317
Number of pages14
JournalExperimental Cell Research
Volume172
Issue number2
DOIs
StatePublished - Oct 1987

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Embryonal Carcinoma Stem Cells
myc Genes
Nerve Tissue
Endoderm
Proteins
DNA-Binding Proteins
Cycloheximide
Transferrin
Growth
Cell Differentiation
Down-Regulation
Insulin
Messenger RNA
Serum
Genes

ASJC Scopus subject areas

  • Cell Biology

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Similarities and differences in the regulation of N-myc and c-myc genes in murine embryonal carcinoma cells. / Sejersen, Thomas; Rahm, Magnus; Szabo, Gabor; Ingvarsson, Sigurdur; Sümegi, Janos.

In: Experimental Cell Research, Vol. 172, No. 2, 10.1987, p. 304-317.

Research output: Contribution to journalArticle

Sejersen, Thomas ; Rahm, Magnus ; Szabo, Gabor ; Ingvarsson, Sigurdur ; Sümegi, Janos. / Similarities and differences in the regulation of N-myc and c-myc genes in murine embryonal carcinoma cells. In: Experimental Cell Research. 1987 ; Vol. 172, No. 2. pp. 304-317.
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