Similar T-cell immune responses induced by group M consensus Env immunogens with wild-type or minimum consensus variable regions

Eric A Weaver, Z. T. Camacho, F. Gao

Research output: Contribution to journalArticle

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Abstract

Consensus HIV-1 genes can decrease the genetic distances between candidate immunogens and field virus strains. To ensure the functionality and optimal presentation of immunologic epitopes, we generated two group-M consensus env genes that contain variable regions either from a wild-type B/C recombinant virus isolate (CON6) or minimal consensus elements (CON-S) in the V1, V2, V4, and V5 regions. C57BL/6 and BALB/c mice were primed twice with CON6, CON-S, and subtype control (92UG37-A and HXB2/Bal-B) DNA and boosted with recombinant vaccinia virus (rVV). Mean antibody titers against 92UG37-A, 89.6-B, 96ZM651-C, CON6, and CON-S Env protein were determined. Both CON6 and CON-S induced higher mean antibody titers against several of the proteins, as compared with the subtype controls. However, no significant differences were found in mean antibody titers in animals immunized with CON6 or CON-S. Cellular immune responses were measured by using five complete Env overlapping peptide sets: subtype A (92UG37-A), subtype B (MN-B, 89.6-B and SF162-B), and subtype C (Chn19-C). The intensity of the induced cellular responses was measured by using pooled Env peptides; T-cell epitopes were identified by using matrix peptide pools and individual peptides. No significant differences in T-cell immune-response intensities were noted between CON6 and CON-S immunized BALB/c and C57BL/6 mice. In BALB/c mice, 10 and eight nonoverlapping T-cell epitopes were identified in CON6 and CON-S, whereas eight epitopes were identified in 92UG37-A and HXB2/BAL-B. In C57BL/6 mice, nine and six nonoverlapping T-cell epitopes were identified after immunization with CON6 and CON-S, respectively, whereas only four and three were identified in 92UG37-A and HXB2/BAL-B, respectively. When combined together from both mouse strains, 18 epitopes were identified. The group M artificial consensus env genes, CON6 and CON-S, were equally immunogenic in breadth and intensity for inducing humoral and cellular immune responses.

Original languageEnglish (US)
Pages (from-to)577-584
Number of pages8
JournalAIDS Research and Human Retroviruses
Volume26
Issue number5
DOIs
StatePublished - May 1 2010

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T-Lymphocyte Epitopes
env Genes
T-Lymphocytes
Dimercaprol
Epitopes
Peptides
Inbred C57BL Mouse
Cellular Immunity
Antibodies
env Gene Products
Viruses
Vaccinia virus
Humoral Immunity
HIV-1
Immunization
DNA
Genes
Proteins

ASJC Scopus subject areas

  • Immunology
  • Virology
  • Infectious Diseases

Cite this

Similar T-cell immune responses induced by group M consensus Env immunogens with wild-type or minimum consensus variable regions. / Weaver, Eric A; Camacho, Z. T.; Gao, F.

In: AIDS Research and Human Retroviruses, Vol. 26, No. 5, 01.05.2010, p. 577-584.

Research output: Contribution to journalArticle

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