Significant role for IRF3 in both T cell and APC effector functions during T cell responses

Zacharey Guinn, Anna T. Lampe, Deborah M Brown, Thomas M Petro

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Interferon Regulatory Factor (IRF)3 is a crucial transcription factor during innate immune responses. Here we show IRF3 also has a role in adaptive T cell immune responses. Expression of IFN-γ, IL-17, and Granzyme B (GrB) during in vitro T cell responses was impaired when either dendritic cells (DCs) or T cells were derived from IRF3KO mice. Unexpectedly, IRF3-dependent NK-activating molecule (INAM), which is an NK cell activating factor of the DC innate immune response, was induced during the T cell response. Additionally, supernatants from responding T cells induced ISG54 in the RAW264.7 macrophage cell line in an IRF3 dependent manner. Moreover, addition of anti-IFN-γ prevented supernatant induction of ISG54 and recombinant IFN-γ stimulated ISG54 expression. Thus, IRF3 in APCs and T cells is required for optimal T-cell effector function and the ability of T cells to influence innate immune function of APCs.

Original languageEnglish (US)
Pages (from-to)141-149
Number of pages9
JournalCellular Immunology
Volume310
DOIs
Publication statusPublished - Dec 1 2016

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Keywords

  • Dendritic cells
  • Granzyme-B
  • Innate immunity
  • Interferon Regulatory Factor-3
  • Interferon stimulated gene-54
  • Interferon-γ
  • Interleukin-17
  • T cells

ASJC Scopus subject areas

  • Immunology

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