Significance of heat-stable and heat-labile enterotoxins in porcine colibacillosis in an additive model for pathogenicity studies

Weiping Zhang, Emil M. Berberov, Jessica Freeling, D. He, Rodney A Moxley, David H. Francis

Research output: Contribution to journalArticle

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Abstract

Although heat-stable (ST) and heat-labile (LT) enterotoxins produced by enterotoxigenic Escherichia coli (ETEC) have been documented as important factors associated with diarrheal diseases, investigations assessing the contributions of individual enterotoxins to the pathogenesis of E. coli infection have been limited. To address the individual roles of enterotoxins in the diarrheal disease caused by K88-positive ETEC in young pigs, enterotoxin-positive and -negative isogenic E. coli strains were constructed by using pBR322 to clone and express LT and STb. Four strains, K88 + astA, K88 + astA/pBR322, K88 + astA STb + , and K88 + astA LT + , were constructed and subsequently included in gnotobiotic piglet challenge studies, and their pathogenesis was assessed. The results indicated that all K88 + isogenic strains were able to colonize the small intestines of piglets exhibiting the K88 receptor. However, only LT- and STb-positive strains caused appreciable diarrhea. Piglets inoculated with the K88 + astA LT + strain became dehydrated within 18 h, while those inoculated with the K88 + astA STb + strain did not, although diarrhea developed in several piglets. The changes in the blood packed-cell volume and plasma total protein of gnotobiotic piglets inoculated with the LT-positive strains were significantly greater than those of pigs inoculated with the K88 astA/pBR322 strain (P = 0.012, P = 0.002). Immunochemistry image analysis also suggested that LT enhanced bacterial colonization in a gnotobiotic piglet model. This investigation suggested that LT is a major contributor to the virulence of K88 + ETEC and that isogenic constructs are a useful tool for studying the pathogenesis of ETEC infection.

Original languageEnglish (US)
Pages (from-to)3107-3114
Number of pages8
JournalInfection and Immunity
Volume74
Issue number6
DOIs
StatePublished - Jun 1 2006

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Enterotoxigenic Escherichia coli
Enterotoxins
Germ-Free Life
Virulence
Swine
Hot Temperature
Escherichia coli Infections
Diarrhea
Immunochemistry
Cell Size
Small Intestine
Blood Proteins
Blood Cells
Clone Cells
Escherichia coli

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Significance of heat-stable and heat-labile enterotoxins in porcine colibacillosis in an additive model for pathogenicity studies. / Zhang, Weiping; Berberov, Emil M.; Freeling, Jessica; He, D.; Moxley, Rodney A; Francis, David H.

In: Infection and Immunity, Vol. 74, No. 6, 01.06.2006, p. 3107-3114.

Research output: Contribution to journalArticle

Zhang, Weiping ; Berberov, Emil M. ; Freeling, Jessica ; He, D. ; Moxley, Rodney A ; Francis, David H. / Significance of heat-stable and heat-labile enterotoxins in porcine colibacillosis in an additive model for pathogenicity studies. In: Infection and Immunity. 2006 ; Vol. 74, No. 6. pp. 3107-3114.
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AB - Although heat-stable (ST) and heat-labile (LT) enterotoxins produced by enterotoxigenic Escherichia coli (ETEC) have been documented as important factors associated with diarrheal diseases, investigations assessing the contributions of individual enterotoxins to the pathogenesis of E. coli infection have been limited. To address the individual roles of enterotoxins in the diarrheal disease caused by K88-positive ETEC in young pigs, enterotoxin-positive and -negative isogenic E. coli strains were constructed by using pBR322 to clone and express LT and STb. Four strains, K88 + astA, K88 + astA/pBR322, K88 + astA STb + , and K88 + astA LT + , were constructed and subsequently included in gnotobiotic piglet challenge studies, and their pathogenesis was assessed. The results indicated that all K88 + isogenic strains were able to colonize the small intestines of piglets exhibiting the K88 receptor. However, only LT- and STb-positive strains caused appreciable diarrhea. Piglets inoculated with the K88 + astA LT + strain became dehydrated within 18 h, while those inoculated with the K88 + astA STb + strain did not, although diarrhea developed in several piglets. The changes in the blood packed-cell volume and plasma total protein of gnotobiotic piglets inoculated with the LT-positive strains were significantly greater than those of pigs inoculated with the K88 astA/pBR322 strain (P = 0.012, P = 0.002). Immunochemistry image analysis also suggested that LT enhanced bacterial colonization in a gnotobiotic piglet model. This investigation suggested that LT is a major contributor to the virulence of K88 + ETEC and that isogenic constructs are a useful tool for studying the pathogenesis of ETEC infection.

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