Shotgun sphingolipid analysis of human aqueous humor

Anna Trzeciecka, Ulises Arbelo, Arturo Barron, Genea Edwards, Sruthi Sampathkumar, Carol Toris, Sanjoy K. Bhattacharya

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)

Abstract

This protocol provides a step-by-step guide to shotgun sphingolipid analysis of aqueous humor. We describe the Bligh and Dyer crude lipid extraction method and electrospray ionization tandem mass spectrometry (ESI-MS/MS) coupled with MZmine 2.21 data processing for identification and ratiometric quantitation of sphingosine, sphingosine-1-phosphate, sphingomyelin, and ceramide.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages97-107
Number of pages11
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1695
ISSN (Print)1064-3745

Fingerprint

Sphingolipids
Sphingosine
Sphingomyelins
Electrospray Ionization Mass Spectrometry
Aqueous Humor
Ceramides
Firearms
Tandem Mass Spectrometry
Lipids
sphingosine 1-phosphate

Keywords

  • Aqueous humor
  • Ceramide
  • Electrospray ionization-tandem mass spectrometry (ESI-MS/MS)
  • MZmine 2.21
  • Shotgun lipidomics
  • Sphingolipids
  • Sphingomyelin
  • Sphingosine
  • Sphingosine-1-phosphate

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Trzeciecka, A., Arbelo, U., Barron, A., Edwards, G., Sampathkumar, S., Toris, C., & Bhattacharya, S. K. (2018). Shotgun sphingolipid analysis of human aqueous humor. In Methods in Molecular Biology (pp. 97-107). (Methods in Molecular Biology; Vol. 1695). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7407-8_10

Shotgun sphingolipid analysis of human aqueous humor. / Trzeciecka, Anna; Arbelo, Ulises; Barron, Arturo; Edwards, Genea; Sampathkumar, Sruthi; Toris, Carol; Bhattacharya, Sanjoy K.

Methods in Molecular Biology. Humana Press Inc., 2018. p. 97-107 (Methods in Molecular Biology; Vol. 1695).

Research output: Chapter in Book/Report/Conference proceedingChapter

Trzeciecka, A, Arbelo, U, Barron, A, Edwards, G, Sampathkumar, S, Toris, C & Bhattacharya, SK 2018, Shotgun sphingolipid analysis of human aqueous humor. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1695, Humana Press Inc., pp. 97-107. https://doi.org/10.1007/978-1-4939-7407-8_10
Trzeciecka A, Arbelo U, Barron A, Edwards G, Sampathkumar S, Toris C et al. Shotgun sphingolipid analysis of human aqueous humor. In Methods in Molecular Biology. Humana Press Inc. 2018. p. 97-107. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-7407-8_10
Trzeciecka, Anna ; Arbelo, Ulises ; Barron, Arturo ; Edwards, Genea ; Sampathkumar, Sruthi ; Toris, Carol ; Bhattacharya, Sanjoy K. / Shotgun sphingolipid analysis of human aqueous humor. Methods in Molecular Biology. Humana Press Inc., 2018. pp. 97-107 (Methods in Molecular Biology).
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