Shotgun proteomic analysis of human-induced sputum

Ben Nicholas, Paul Skipp, Richard Mould, Stephen Israel Rennard, Donna E. Davies, C. David O'Connor, Ratko Djukanović

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

Induced sputum is a readily accessible biological fluid whose composition may alter as a consequence of disease. To date, however, the proteins that routinely populate this biofluid are largely unknown, in part due to the technical difficulties in processing such mucin-rich samples. To provide a catalogue of sputum proteins, we have surveyed the proteome of human-induced sputum (sputome). A combination of 2-D gel analysis and GeLC-MS/MS allowed a total of 191 human proteins to be confidently assigned. In addition to the expected components, several hitherto unreported proteins were found to be present, including three members of the annexin family, kallikreins 1 and 11, and peroxiredoxins 1, 2 and 5. Other sets of proteins identified included four proteins previously annotated as hypothetical or conserved hypothetical. Taken together, these data represent the first extensive survey of the proteome of induced sputum and provide a platform for future identification of biomarkers of lung disease.

Original languageEnglish (US)
Pages (from-to)4390-4401
Number of pages12
JournalProteomics
Volume6
Issue number15
DOIs
StatePublished - Aug 1 2006

Fingerprint

Firearms
Sputum
Proteomics
Proteins
Proteome
Peroxiredoxins
Tissue Kallikreins
Annexins
Pulmonary diseases
Biomarkers
Mucins
Lung Diseases
Gels
Fluids
Processing
Chemical analysis

Keywords

  • 2-D PAGE
  • GeLC-MS/MS
  • Human bronchoalveolar lavage fluid proteins
  • Induced sputum

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Nicholas, B., Skipp, P., Mould, R., Rennard, S. I., Davies, D. E., O'Connor, C. D., & Djukanović, R. (2006). Shotgun proteomic analysis of human-induced sputum. Proteomics, 6(15), 4390-4401. https://doi.org/10.1002/pmic.200600011

Shotgun proteomic analysis of human-induced sputum. / Nicholas, Ben; Skipp, Paul; Mould, Richard; Rennard, Stephen Israel; Davies, Donna E.; O'Connor, C. David; Djukanović, Ratko.

In: Proteomics, Vol. 6, No. 15, 01.08.2006, p. 4390-4401.

Research output: Contribution to journalArticle

Nicholas, B, Skipp, P, Mould, R, Rennard, SI, Davies, DE, O'Connor, CD & Djukanović, R 2006, 'Shotgun proteomic analysis of human-induced sputum', Proteomics, vol. 6, no. 15, pp. 4390-4401. https://doi.org/10.1002/pmic.200600011
Nicholas B, Skipp P, Mould R, Rennard SI, Davies DE, O'Connor CD et al. Shotgun proteomic analysis of human-induced sputum. Proteomics. 2006 Aug 1;6(15):4390-4401. https://doi.org/10.1002/pmic.200600011
Nicholas, Ben ; Skipp, Paul ; Mould, Richard ; Rennard, Stephen Israel ; Davies, Donna E. ; O'Connor, C. David ; Djukanović, Ratko. / Shotgun proteomic analysis of human-induced sputum. In: Proteomics. 2006 ; Vol. 6, No. 15. pp. 4390-4401.
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