Shot-gun sequencing strategy for long-range genome mapping: A pilot study

Eugene R. Zabarovsky, Vladimir I. Kashuba, Bertil Pettersson, Nikolai Petrov, Vladimir Zakharyev, Rinat Gizatullin, Tatyana Lebedeva, George Klein, Ekaterina S. Pokrovskaya, Veronika I. Zabarovska, Rando Allikmets, Rikard Erlandsson, Dmitry Domninsky, Janos Sumegi, Eric J. Stanbridge, Gösta Winberg, Mathias Uhlen

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

We have recently proposed a strategy for construction of long-range physical maps based on random sequencing of NotI linking and jumping clones. Here, we present results of sequence comparison between 168 NotI linking (100 of them were sequenced from both sides) and 81 chromosome 3-specific jumping clones. We were able to identify 14 NotI jumping clones (17%), each joined with two NotI linking clones. The average size of chromosomal jumps was about 650 kb. The assembled 42 NotI genomic fragments correspond to 12-15% of chromosome 3. These results demonstrate the value of random sequencing of NotI linking and jumping clones for genome mapping. This mapping proposal can be used for connecting physical and genetic maps of the human genome and will be a valuable supplement to YAC and cosmid library based mapping projects.

Original languageEnglish (US)
Pages (from-to)495-500
Number of pages6
JournalGenomics
Volume21
Issue number3
DOIs
StatePublished - Jun 1994

Fingerprint

Chromosome Mapping
Firearms
Clone Cells
Chromosomes, Human, Pair 3
Cosmids
Human Genome

ASJC Scopus subject areas

  • Genetics

Cite this

Zabarovsky, E. R., Kashuba, V. I., Pettersson, B., Petrov, N., Zakharyev, V., Gizatullin, R., ... Uhlen, M. (1994). Shot-gun sequencing strategy for long-range genome mapping: A pilot study. Genomics, 21(3), 495-500. https://doi.org/10.1006/geno.1994.1307

Shot-gun sequencing strategy for long-range genome mapping : A pilot study. / Zabarovsky, Eugene R.; Kashuba, Vladimir I.; Pettersson, Bertil; Petrov, Nikolai; Zakharyev, Vladimir; Gizatullin, Rinat; Lebedeva, Tatyana; Klein, George; Pokrovskaya, Ekaterina S.; Zabarovska, Veronika I.; Allikmets, Rando; Erlandsson, Rikard; Domninsky, Dmitry; Sumegi, Janos; Stanbridge, Eric J.; Winberg, Gösta; Uhlen, Mathias.

In: Genomics, Vol. 21, No. 3, 06.1994, p. 495-500.

Research output: Contribution to journalArticle

Zabarovsky, ER, Kashuba, VI, Pettersson, B, Petrov, N, Zakharyev, V, Gizatullin, R, Lebedeva, T, Klein, G, Pokrovskaya, ES, Zabarovska, VI, Allikmets, R, Erlandsson, R, Domninsky, D, Sumegi, J, Stanbridge, EJ, Winberg, G & Uhlen, M 1994, 'Shot-gun sequencing strategy for long-range genome mapping: A pilot study', Genomics, vol. 21, no. 3, pp. 495-500. https://doi.org/10.1006/geno.1994.1307
Zabarovsky ER, Kashuba VI, Pettersson B, Petrov N, Zakharyev V, Gizatullin R et al. Shot-gun sequencing strategy for long-range genome mapping: A pilot study. Genomics. 1994 Jun;21(3):495-500. https://doi.org/10.1006/geno.1994.1307
Zabarovsky, Eugene R. ; Kashuba, Vladimir I. ; Pettersson, Bertil ; Petrov, Nikolai ; Zakharyev, Vladimir ; Gizatullin, Rinat ; Lebedeva, Tatyana ; Klein, George ; Pokrovskaya, Ekaterina S. ; Zabarovska, Veronika I. ; Allikmets, Rando ; Erlandsson, Rikard ; Domninsky, Dmitry ; Sumegi, Janos ; Stanbridge, Eric J. ; Winberg, Gösta ; Uhlen, Mathias. / Shot-gun sequencing strategy for long-range genome mapping : A pilot study. In: Genomics. 1994 ; Vol. 21, No. 3. pp. 495-500.
@article{05a8c1e331f9403099fa772e3f32d128,
title = "Shot-gun sequencing strategy for long-range genome mapping: A pilot study",
abstract = "We have recently proposed a strategy for construction of long-range physical maps based on random sequencing of NotI linking and jumping clones. Here, we present results of sequence comparison between 168 NotI linking (100 of them were sequenced from both sides) and 81 chromosome 3-specific jumping clones. We were able to identify 14 NotI jumping clones (17{\%}), each joined with two NotI linking clones. The average size of chromosomal jumps was about 650 kb. The assembled 42 NotI genomic fragments correspond to 12-15{\%} of chromosome 3. These results demonstrate the value of random sequencing of NotI linking and jumping clones for genome mapping. This mapping proposal can be used for connecting physical and genetic maps of the human genome and will be a valuable supplement to YAC and cosmid library based mapping projects.",
author = "Zabarovsky, {Eugene R.} and Kashuba, {Vladimir I.} and Bertil Pettersson and Nikolai Petrov and Vladimir Zakharyev and Rinat Gizatullin and Tatyana Lebedeva and George Klein and Pokrovskaya, {Ekaterina S.} and Zabarovska, {Veronika I.} and Rando Allikmets and Rikard Erlandsson and Dmitry Domninsky and Janos Sumegi and Stanbridge, {Eric J.} and G{\"o}sta Winberg and Mathias Uhlen",
year = "1994",
month = "6",
doi = "10.1006/geno.1994.1307",
language = "English (US)",
volume = "21",
pages = "495--500",
journal = "Genomics",
issn = "0888-7543",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - Shot-gun sequencing strategy for long-range genome mapping

T2 - A pilot study

AU - Zabarovsky, Eugene R.

AU - Kashuba, Vladimir I.

AU - Pettersson, Bertil

AU - Petrov, Nikolai

AU - Zakharyev, Vladimir

AU - Gizatullin, Rinat

AU - Lebedeva, Tatyana

AU - Klein, George

AU - Pokrovskaya, Ekaterina S.

AU - Zabarovska, Veronika I.

AU - Allikmets, Rando

AU - Erlandsson, Rikard

AU - Domninsky, Dmitry

AU - Sumegi, Janos

AU - Stanbridge, Eric J.

AU - Winberg, Gösta

AU - Uhlen, Mathias

PY - 1994/6

Y1 - 1994/6

N2 - We have recently proposed a strategy for construction of long-range physical maps based on random sequencing of NotI linking and jumping clones. Here, we present results of sequence comparison between 168 NotI linking (100 of them were sequenced from both sides) and 81 chromosome 3-specific jumping clones. We were able to identify 14 NotI jumping clones (17%), each joined with two NotI linking clones. The average size of chromosomal jumps was about 650 kb. The assembled 42 NotI genomic fragments correspond to 12-15% of chromosome 3. These results demonstrate the value of random sequencing of NotI linking and jumping clones for genome mapping. This mapping proposal can be used for connecting physical and genetic maps of the human genome and will be a valuable supplement to YAC and cosmid library based mapping projects.

AB - We have recently proposed a strategy for construction of long-range physical maps based on random sequencing of NotI linking and jumping clones. Here, we present results of sequence comparison between 168 NotI linking (100 of them were sequenced from both sides) and 81 chromosome 3-specific jumping clones. We were able to identify 14 NotI jumping clones (17%), each joined with two NotI linking clones. The average size of chromosomal jumps was about 650 kb. The assembled 42 NotI genomic fragments correspond to 12-15% of chromosome 3. These results demonstrate the value of random sequencing of NotI linking and jumping clones for genome mapping. This mapping proposal can be used for connecting physical and genetic maps of the human genome and will be a valuable supplement to YAC and cosmid library based mapping projects.

UR - http://www.scopus.com/inward/record.url?scp=0028339338&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028339338&partnerID=8YFLogxK

U2 - 10.1006/geno.1994.1307

DO - 10.1006/geno.1994.1307

M3 - Article

C2 - 7959725

AN - SCOPUS:0028339338

VL - 21

SP - 495

EP - 500

JO - Genomics

JF - Genomics

SN - 0888-7543

IS - 3

ER -