Short‐term primary culture of rat prostate tumor epithelial cells on reconstituted basement membrane as a useful in vitro model to assess drug action on prostatic cancer: Efficacy of the thiazolidinedione derivative CGP 19984

Wendy K. Shea, Jennifer D Black, Margot M. Ip

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A model system has been developed that permits short‐term culture of rat R3327 prostate adenocarcinoma epithelial cells on a reconstituted basement membrane. Growth of prostate tumor cells under these conditions resulted in an enriched epithelial cell population that exhibited an eightfold increase in cell number in 10 days. This model system was used to test the efficacy of the thiazolidinedione derivative CGP 19984, a drug that inhibits luteinizing hormone secretion in vivo. At concentrations ranging between 1 and 25 μg/ml, CGP 19984 inhibited growth of the prostate tumor epithelial cells in a dose‐dependent manner. The results thus demonstrate a direct effect of CGP 19984, which complements its indirect antitumor action in vivo, and suggest that this drug might be an effective agent for treatment of prostatic cancer. Moreover, growth of prostate tumor epithelial cells on a reconstituted basement membrane provides a useful system for in vitro testing of drugs for prostate cancer.

Original languageEnglish (US)
Pages (from-to)157-170
Number of pages14
JournalThe Prostate
Volume15
Issue number2
DOIs
StatePublished - Jan 1 1989

Fingerprint

Basement Membrane
Prostate
Prostatic Neoplasms
Epithelial Cells
Pharmaceutical Preparations
Neoplasms
Growth
Luteinizing Hormone
Adenocarcinoma
Cell Count
2,4-thiazolidinedione
In Vitro Techniques
CGP 19984
Population

Keywords

  • Iuteinizing hormone
  • R3327 prostate tumor
  • cell proliferation
  • drug efficacy
  • extracellular matrix

ASJC Scopus subject areas

  • Oncology
  • Urology

Cite this

@article{1e7a4fcbb8b84c098060528c0cf3cd38,
title = "Short‐term primary culture of rat prostate tumor epithelial cells on reconstituted basement membrane as a useful in vitro model to assess drug action on prostatic cancer: Efficacy of the thiazolidinedione derivative CGP 19984",
abstract = "A model system has been developed that permits short‐term culture of rat R3327 prostate adenocarcinoma epithelial cells on a reconstituted basement membrane. Growth of prostate tumor cells under these conditions resulted in an enriched epithelial cell population that exhibited an eightfold increase in cell number in 10 days. This model system was used to test the efficacy of the thiazolidinedione derivative CGP 19984, a drug that inhibits luteinizing hormone secretion in vivo. At concentrations ranging between 1 and 25 μg/ml, CGP 19984 inhibited growth of the prostate tumor epithelial cells in a dose‐dependent manner. The results thus demonstrate a direct effect of CGP 19984, which complements its indirect antitumor action in vivo, and suggest that this drug might be an effective agent for treatment of prostatic cancer. Moreover, growth of prostate tumor epithelial cells on a reconstituted basement membrane provides a useful system for in vitro testing of drugs for prostate cancer.",
keywords = "Iuteinizing hormone, R3327 prostate tumor, cell proliferation, drug efficacy, extracellular matrix",
author = "Shea, {Wendy K.} and Black, {Jennifer D} and Ip, {Margot M.}",
year = "1989",
month = "1",
day = "1",
doi = "10.1002/pros.2990150209",
language = "English (US)",
volume = "15",
pages = "157--170",
journal = "Prostate",
issn = "0270-4137",
publisher = "Wiley-Liss Inc.",
number = "2",

}

TY - JOUR

T1 - Short‐term primary culture of rat prostate tumor epithelial cells on reconstituted basement membrane as a useful in vitro model to assess drug action on prostatic cancer

T2 - Efficacy of the thiazolidinedione derivative CGP 19984

AU - Shea, Wendy K.

AU - Black, Jennifer D

AU - Ip, Margot M.

PY - 1989/1/1

Y1 - 1989/1/1

N2 - A model system has been developed that permits short‐term culture of rat R3327 prostate adenocarcinoma epithelial cells on a reconstituted basement membrane. Growth of prostate tumor cells under these conditions resulted in an enriched epithelial cell population that exhibited an eightfold increase in cell number in 10 days. This model system was used to test the efficacy of the thiazolidinedione derivative CGP 19984, a drug that inhibits luteinizing hormone secretion in vivo. At concentrations ranging between 1 and 25 μg/ml, CGP 19984 inhibited growth of the prostate tumor epithelial cells in a dose‐dependent manner. The results thus demonstrate a direct effect of CGP 19984, which complements its indirect antitumor action in vivo, and suggest that this drug might be an effective agent for treatment of prostatic cancer. Moreover, growth of prostate tumor epithelial cells on a reconstituted basement membrane provides a useful system for in vitro testing of drugs for prostate cancer.

AB - A model system has been developed that permits short‐term culture of rat R3327 prostate adenocarcinoma epithelial cells on a reconstituted basement membrane. Growth of prostate tumor cells under these conditions resulted in an enriched epithelial cell population that exhibited an eightfold increase in cell number in 10 days. This model system was used to test the efficacy of the thiazolidinedione derivative CGP 19984, a drug that inhibits luteinizing hormone secretion in vivo. At concentrations ranging between 1 and 25 μg/ml, CGP 19984 inhibited growth of the prostate tumor epithelial cells in a dose‐dependent manner. The results thus demonstrate a direct effect of CGP 19984, which complements its indirect antitumor action in vivo, and suggest that this drug might be an effective agent for treatment of prostatic cancer. Moreover, growth of prostate tumor epithelial cells on a reconstituted basement membrane provides a useful system for in vitro testing of drugs for prostate cancer.

KW - Iuteinizing hormone

KW - R3327 prostate tumor

KW - cell proliferation

KW - drug efficacy

KW - extracellular matrix

UR - http://www.scopus.com/inward/record.url?scp=0024460071&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024460071&partnerID=8YFLogxK

U2 - 10.1002/pros.2990150209

DO - 10.1002/pros.2990150209

M3 - Article

C2 - 2798233

AN - SCOPUS:0024460071

VL - 15

SP - 157

EP - 170

JO - Prostate

JF - Prostate

SN - 0270-4137

IS - 2

ER -