Sex differences in neurotensin and substance P following nicotine self-administration in rats

Steven T. Pittenger, Natashia Swalve, Shinnyi Chou, Misty D. Smith, Amanda J. Hoonakker, Cindy M. Pudiak, Annette E. Fleckenstein, Glen R. Hanson, Rick A Bevins

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Investigator-administered nicotine alters neurotensin and substance P levels in Sprague-Dawley rats. This finding suggested a role of the dopamine-related endogenous neuropeptides in nicotine addiction. We sought to extend this observation by determining the responses of neurotensin and substance P systems (assessed using radioimmunoassay) in male and female rats following nicotine self-administration (SA). Male and female Sprague-Dawley were trained to self-administer nicotine, or receive saline infusions yoked to a nicotine-administering rat during daily sessions (1-h; 21 days). Brains were extracted 3 h after the last SA session. Nicotine SA increased tissue levels of neurotensin in the males in the anterior and posterior caudate, globus pallidus, frontal cortex, nucleus accumbens core and shell, and ventral tegmental area. Nicotine SA also increased tissue levels of neurotensin in the females in the anterior caudate, globus pallidus, nucleus accumbens core and shell, but not in the posterior caudate, frontal cortex, or ventral tegmental area. There were fewer sex differences observed in the substance P systems. Nicotine SA increased tissue levels of substance P in both the males and females in the posterior caudate, globus pallidus, frontal cortex, nucleus accumbens shell, and ventral tegmental area. A sex difference was observed in the nucleus accumbens core, where nicotine SA increased tissue levels of substance P in the males, yet decreased levels in the females. The regulation of neuropeptides following nicotine SA may play a role in the susceptibility to nicotine dependence in females and males. Synapse 70:336–346, 2016.

Original languageEnglish (US)
Pages (from-to)336-346
Number of pages11
JournalSynapse
Volume70
Issue number8
DOIs
StatePublished - Aug 1 2016

Fingerprint

Neurotensin
Self Administration
Substance P
Nicotine
Sex Characteristics
Nucleus Accumbens
Ventral Tegmental Area
Globus Pallidus
Frontal Lobe
Neuropeptides
Tobacco Use Disorder
Synapses
Radioimmunoassay
Sprague Dawley Rats
Dopamine
Research Personnel

Keywords

  • addiction
  • dopamine
  • female
  • limbic-related
  • male
  • neuropeptide

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Cite this

Pittenger, S. T., Swalve, N., Chou, S., Smith, M. D., Hoonakker, A. J., Pudiak, C. M., ... Bevins, R. A. (2016). Sex differences in neurotensin and substance P following nicotine self-administration in rats. Synapse, 70(8), 336-346. https://doi.org/10.1002/syn.21907

Sex differences in neurotensin and substance P following nicotine self-administration in rats. / Pittenger, Steven T.; Swalve, Natashia; Chou, Shinnyi; Smith, Misty D.; Hoonakker, Amanda J.; Pudiak, Cindy M.; Fleckenstein, Annette E.; Hanson, Glen R.; Bevins, Rick A.

In: Synapse, Vol. 70, No. 8, 01.08.2016, p. 336-346.

Research output: Contribution to journalArticle

Pittenger, ST, Swalve, N, Chou, S, Smith, MD, Hoonakker, AJ, Pudiak, CM, Fleckenstein, AE, Hanson, GR & Bevins, RA 2016, 'Sex differences in neurotensin and substance P following nicotine self-administration in rats', Synapse, vol. 70, no. 8, pp. 336-346. https://doi.org/10.1002/syn.21907
Pittenger ST, Swalve N, Chou S, Smith MD, Hoonakker AJ, Pudiak CM et al. Sex differences in neurotensin and substance P following nicotine self-administration in rats. Synapse. 2016 Aug 1;70(8):336-346. https://doi.org/10.1002/syn.21907
Pittenger, Steven T. ; Swalve, Natashia ; Chou, Shinnyi ; Smith, Misty D. ; Hoonakker, Amanda J. ; Pudiak, Cindy M. ; Fleckenstein, Annette E. ; Hanson, Glen R. ; Bevins, Rick A. / Sex differences in neurotensin and substance P following nicotine self-administration in rats. In: Synapse. 2016 ; Vol. 70, No. 8. pp. 336-346.
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abstract = "Investigator-administered nicotine alters neurotensin and substance P levels in Sprague-Dawley rats. This finding suggested a role of the dopamine-related endogenous neuropeptides in nicotine addiction. We sought to extend this observation by determining the responses of neurotensin and substance P systems (assessed using radioimmunoassay) in male and female rats following nicotine self-administration (SA). Male and female Sprague-Dawley were trained to self-administer nicotine, or receive saline infusions yoked to a nicotine-administering rat during daily sessions (1-h; 21 days). Brains were extracted 3 h after the last SA session. Nicotine SA increased tissue levels of neurotensin in the males in the anterior and posterior caudate, globus pallidus, frontal cortex, nucleus accumbens core and shell, and ventral tegmental area. Nicotine SA also increased tissue levels of neurotensin in the females in the anterior caudate, globus pallidus, nucleus accumbens core and shell, but not in the posterior caudate, frontal cortex, or ventral tegmental area. There were fewer sex differences observed in the substance P systems. Nicotine SA increased tissue levels of substance P in both the males and females in the posterior caudate, globus pallidus, frontal cortex, nucleus accumbens shell, and ventral tegmental area. A sex difference was observed in the nucleus accumbens core, where nicotine SA increased tissue levels of substance P in the males, yet decreased levels in the females. The regulation of neuropeptides following nicotine SA may play a role in the susceptibility to nicotine dependence in females and males. Synapse 70:336–346, 2016.",
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