Serum antibody responses in human periodontitis to cellular components of Capnocytophaga

J. K. Dyer, Richard A Reinhardt, Thomas M Petro, E. A. Strom

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6 Scopus citations

Abstract

Serum levels of IgM, IgG and IgG-antibody subclasses directed against cell envelopes, lipopolysaccharides and cytoplasmic fractions from Capnocytophaga sputigena, C. gingivalis and C. ochracea were examined in age-, race- and sex-matched periodontally healthy (n = 25) subjects and subjects with adult periodontitis (n = 25). The envelopes and cytoplasmic fractions were obtained by ballistic disintegration of the cells and ultracentrifugation. Cell envelopes were treated with DNase, RNase and lysozyme. Lipopolysaccharides were obtained by hot phenol-water extraction and treated with DNase and RNase. The relative levels of the antibodies in response to the cell fractions were measured by the streptavidinbiotin micro enzyme-linked immunosorbent assay. Both groups showed IgM and IgG antibodies to each fraction of the three Capnocytophaga species, but the frequency of positive IgG subclass responses varied. The IgG4 responses were lower than the other subclasses. There were no significant differences between the IgM antibody levels of the two groups. However, the adult periodontitis group had significantly lower IgG antibody titres to the cell envelopes and cytoplasmic fractions of C. gingivalis and C. ochracea, and lipopolysaccharide of C. gingivalis. These results were reflected in the depressed levels of IgG1 and/or IgG2 to these cellular fractions from the same bacterial species. The adult periodontitis group also showed a lower level of IgG1 to the cytoplasmic fractions of C. sputigena without any depression in the total IgG antibody level. There were no significant differences between the groups in IgG3 and IgG4 antibody levels to any of the cellular fractions. These findings suggest that some individuals are unable to mount certain IgG subclass responses to Capnocytophaga, perhaps increasing their susceptibility to periodontitis, and or may indicate the presence of a smaller population of Capnocytophaga at the diseased sites.

Original languageEnglish (US)
Pages (from-to)725-731
Number of pages7
JournalArchives of Oral Biology
Volume37
Issue number9
DOIs
StatePublished - Sep 1992

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Keywords

  • Capnocytophaga
  • IgG
  • IgG subclasses
  • IgM
  • cell envelope
  • cytoplasmic fraction
  • lipopolysaccharide
  • serum antibodies

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Dentistry(all)
  • Cell Biology

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