Serological & molecular approaches for diagnosis of leptospirosis in a tertiary care hospital in North India: A 10-year study

Rama Chaudhry, A. Das, M. M. Premlatha, A. Choudhary, B. K. Chourasia, D. S. Chandel, A. B. Dey

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Background & objectives: Leptospirosis, a spirochetal zoonosis, is underreported from the northern States of India. This study reports results of a 10-year retrospective sero-epidemiological survey of leptospirosis conducted in a tertiary care hospital in New Delhi, India. Method: A total of 1453 patients clinically suspected for leptospirosis were included and investigated initially by IgM ELISA. A proportion of these were subjected to culture, microscopic agglutination test (MAT) and polymerase chain reaction (PCR). Results: Of the 1453 patients, 391 (26.90%) were positive serologically by IgM ELISA. Seropositive and seronegative patients revealed no significant difference in clinical features and laboratory parameters. Amongst the IgM seropositive cases, culture for leptospires was positive in 5 of 192 (2.6%), MAT in 50 of 138 (36.23%), PCR from blood and urine in 10 of 115 (8.7%) and 10 of 38 (26.31%) cases, respectively. In Leptospira spp. positive patients co-infections with viral hepatitis E, malaria and dengue fever were diagnosed in 27 cases. Interpretation & conclusions: The overall seropositivity for leptospirosis was 26.9 per cent in our study. A decreasing trend in seropositivity was observed in recent years. Co-infections with malaria, dengue, hepatitis A and E were also seen. Since leptospirosis is a treatable disease, correct and rapid diagnosis may help in effective management of patients.

Original languageEnglish (US)
Pages (from-to)785-790
Number of pages6
JournalIndian Journal of Medical Research
Volume137
Issue number4
StatePublished - Apr 1 2013

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Leptospirosis
antineoplaston A10
Tertiary Healthcare
Tertiary Care Centers
Immunoglobulin M
India
Polymerase chain reaction
Hepatitis E
Agglutination Tests
Dengue
Coinfection
Malaria
Enzyme-Linked Immunosorbent Assay
Blood
Leptospira
Polymerase Chain Reaction
Hepatitis A
Zoonoses
Urine

Keywords

  • Co-infection
  • Delhi
  • IgM ELISA
  • Leptospirosis
  • MAT
  • PCR
  • Sero-epidemiology

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Chaudhry, R., Das, A., Premlatha, M. M., Choudhary, A., Chourasia, B. K., Chandel, D. S., & Dey, A. B. (2013). Serological & molecular approaches for diagnosis of leptospirosis in a tertiary care hospital in North India: A 10-year study. Indian Journal of Medical Research, 137(4), 785-790.

Serological & molecular approaches for diagnosis of leptospirosis in a tertiary care hospital in North India : A 10-year study. / Chaudhry, Rama; Das, A.; Premlatha, M. M.; Choudhary, A.; Chourasia, B. K.; Chandel, D. S.; Dey, A. B.

In: Indian Journal of Medical Research, Vol. 137, No. 4, 01.04.2013, p. 785-790.

Research output: Contribution to journalArticle

Chaudhry, R, Das, A, Premlatha, MM, Choudhary, A, Chourasia, BK, Chandel, DS & Dey, AB 2013, 'Serological & molecular approaches for diagnosis of leptospirosis in a tertiary care hospital in North India: A 10-year study', Indian Journal of Medical Research, vol. 137, no. 4, pp. 785-790.
Chaudhry R, Das A, Premlatha MM, Choudhary A, Chourasia BK, Chandel DS et al. Serological & molecular approaches for diagnosis of leptospirosis in a tertiary care hospital in North India: A 10-year study. Indian Journal of Medical Research. 2013 Apr 1;137(4):785-790.
Chaudhry, Rama ; Das, A. ; Premlatha, M. M. ; Choudhary, A. ; Chourasia, B. K. ; Chandel, D. S. ; Dey, A. B. / Serological & molecular approaches for diagnosis of leptospirosis in a tertiary care hospital in North India : A 10-year study. In: Indian Journal of Medical Research. 2013 ; Vol. 137, No. 4. pp. 785-790.
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abstract = "Background & objectives: Leptospirosis, a spirochetal zoonosis, is underreported from the northern States of India. This study reports results of a 10-year retrospective sero-epidemiological survey of leptospirosis conducted in a tertiary care hospital in New Delhi, India. Method: A total of 1453 patients clinically suspected for leptospirosis were included and investigated initially by IgM ELISA. A proportion of these were subjected to culture, microscopic agglutination test (MAT) and polymerase chain reaction (PCR). Results: Of the 1453 patients, 391 (26.90{\%}) were positive serologically by IgM ELISA. Seropositive and seronegative patients revealed no significant difference in clinical features and laboratory parameters. Amongst the IgM seropositive cases, culture for leptospires was positive in 5 of 192 (2.6{\%}), MAT in 50 of 138 (36.23{\%}), PCR from blood and urine in 10 of 115 (8.7{\%}) and 10 of 38 (26.31{\%}) cases, respectively. In Leptospira spp. positive patients co-infections with viral hepatitis E, malaria and dengue fever were diagnosed in 27 cases. Interpretation & conclusions: The overall seropositivity for leptospirosis was 26.9 per cent in our study. A decreasing trend in seropositivity was observed in recent years. Co-infections with malaria, dengue, hepatitis A and E were also seen. Since leptospirosis is a treatable disease, correct and rapid diagnosis may help in effective management of patients.",
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