Serodiagnosis of acute typhoid fever in nigerian pediatric cases by detection of serum IgA and IgG against hemolysin e and Lipopolysaccharide

D. Huw Davies, Aarti Jain, Rie Nakajima, Li Liang, Algis Jasinskis, Medalyn Supnet, Philip L. Felgner, Andy Teng, Jozelyn Pablo, Douglas M. Molina, Stephen K Obaro

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Inexpensive, easy-to-use, and highly sensitive diagnostic tests are currently unavailable for typhoid fever. To identify candidate serodiagnostic markers, we have probed microarrays displaying the full Salmonella enterica serovar Typhi (S. Typhi) proteome of 4,352 different proteins + lipopolysaccharides (LPSs), with sera from Nigerian pediatric typhoid and other febrile cases, Nigerian healthy controls, and healthy U.S. adults. Nigerian antibody profiles were broad (∼500 seropositive antigens) and mainly low level, with a small number of stronger "hits," whereas the profile in U.S. adults was < 1/5 as broad, consistent with endemic exposure in Nigeria. Nigerian profiles were largely unaffected by clinical diagnosis, although the response against t1477 (hemolysin E) consistently emerged as stronger in typhoid cases. The response to LPS was also a strong discriminator of healthy controls and typhoid, although LPS did not discriminate between typhoid and nontyphoidal Salmonella (NTS) disease. As a first step toward the development of a point-of-care diagnostic, t1477 and LPS were evaluated on immunostrips. Both provided good discrimination between healthy controls and typhoid/NTS disease. Such a test could provide a useful screen for salmonellosis (typhoid and NTS disease) in suspected pediatric cases that present with undefined febrile disease.

Original languageEnglish (US)
Pages (from-to)431-439
Number of pages9
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume95
Issue number2
DOIs
StatePublished - Aug 1 2016

Fingerprint

Hemolysin Proteins
Typhoid Fever
Serologic Tests
Immunoglobulin A
Lipopolysaccharides
Immunoglobulin G
Pediatrics
Serum
Salmonella
Point-of-Care Systems
Salmonella typhi
Salmonella Infections
Proteome
Nigeria
Routine Diagnostic Tests
Fever
Antigens
Antibodies

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases
  • Virology

Cite this

Serodiagnosis of acute typhoid fever in nigerian pediatric cases by detection of serum IgA and IgG against hemolysin e and Lipopolysaccharide. / Davies, D. Huw; Jain, Aarti; Nakajima, Rie; Liang, Li; Jasinskis, Algis; Supnet, Medalyn; Felgner, Philip L.; Teng, Andy; Pablo, Jozelyn; Molina, Douglas M.; Obaro, Stephen K.

In: American Journal of Tropical Medicine and Hygiene, Vol. 95, No. 2, 01.08.2016, p. 431-439.

Research output: Contribution to journalArticle

Davies, D. Huw ; Jain, Aarti ; Nakajima, Rie ; Liang, Li ; Jasinskis, Algis ; Supnet, Medalyn ; Felgner, Philip L. ; Teng, Andy ; Pablo, Jozelyn ; Molina, Douglas M. ; Obaro, Stephen K. / Serodiagnosis of acute typhoid fever in nigerian pediatric cases by detection of serum IgA and IgG against hemolysin e and Lipopolysaccharide. In: American Journal of Tropical Medicine and Hygiene. 2016 ; Vol. 95, No. 2. pp. 431-439.
@article{2c0a371d955a4b47bd6c9b0baa7ccb58,
title = "Serodiagnosis of acute typhoid fever in nigerian pediatric cases by detection of serum IgA and IgG against hemolysin e and Lipopolysaccharide",
abstract = "Inexpensive, easy-to-use, and highly sensitive diagnostic tests are currently unavailable for typhoid fever. To identify candidate serodiagnostic markers, we have probed microarrays displaying the full Salmonella enterica serovar Typhi (S. Typhi) proteome of 4,352 different proteins + lipopolysaccharides (LPSs), with sera from Nigerian pediatric typhoid and other febrile cases, Nigerian healthy controls, and healthy U.S. adults. Nigerian antibody profiles were broad (∼500 seropositive antigens) and mainly low level, with a small number of stronger {"}hits,{"} whereas the profile in U.S. adults was < 1/5 as broad, consistent with endemic exposure in Nigeria. Nigerian profiles were largely unaffected by clinical diagnosis, although the response against t1477 (hemolysin E) consistently emerged as stronger in typhoid cases. The response to LPS was also a strong discriminator of healthy controls and typhoid, although LPS did not discriminate between typhoid and nontyphoidal Salmonella (NTS) disease. As a first step toward the development of a point-of-care diagnostic, t1477 and LPS were evaluated on immunostrips. Both provided good discrimination between healthy controls and typhoid/NTS disease. Such a test could provide a useful screen for salmonellosis (typhoid and NTS disease) in suspected pediatric cases that present with undefined febrile disease.",
author = "Davies, {D. Huw} and Aarti Jain and Rie Nakajima and Li Liang and Algis Jasinskis and Medalyn Supnet and Felgner, {Philip L.} and Andy Teng and Jozelyn Pablo and Molina, {Douglas M.} and Obaro, {Stephen K}",
year = "2016",
month = "8",
day = "1",
doi = "10.4269/ajtmh.15-0869",
language = "English (US)",
volume = "95",
pages = "431--439",
journal = "American Journal of Tropical Medicine and Hygiene",
issn = "0002-9637",
publisher = "American Society of Tropical Medicine and Hygiene",
number = "2",

}

TY - JOUR

T1 - Serodiagnosis of acute typhoid fever in nigerian pediatric cases by detection of serum IgA and IgG against hemolysin e and Lipopolysaccharide

AU - Davies, D. Huw

AU - Jain, Aarti

AU - Nakajima, Rie

AU - Liang, Li

AU - Jasinskis, Algis

AU - Supnet, Medalyn

AU - Felgner, Philip L.

AU - Teng, Andy

AU - Pablo, Jozelyn

AU - Molina, Douglas M.

AU - Obaro, Stephen K

PY - 2016/8/1

Y1 - 2016/8/1

N2 - Inexpensive, easy-to-use, and highly sensitive diagnostic tests are currently unavailable for typhoid fever. To identify candidate serodiagnostic markers, we have probed microarrays displaying the full Salmonella enterica serovar Typhi (S. Typhi) proteome of 4,352 different proteins + lipopolysaccharides (LPSs), with sera from Nigerian pediatric typhoid and other febrile cases, Nigerian healthy controls, and healthy U.S. adults. Nigerian antibody profiles were broad (∼500 seropositive antigens) and mainly low level, with a small number of stronger "hits," whereas the profile in U.S. adults was < 1/5 as broad, consistent with endemic exposure in Nigeria. Nigerian profiles were largely unaffected by clinical diagnosis, although the response against t1477 (hemolysin E) consistently emerged as stronger in typhoid cases. The response to LPS was also a strong discriminator of healthy controls and typhoid, although LPS did not discriminate between typhoid and nontyphoidal Salmonella (NTS) disease. As a first step toward the development of a point-of-care diagnostic, t1477 and LPS were evaluated on immunostrips. Both provided good discrimination between healthy controls and typhoid/NTS disease. Such a test could provide a useful screen for salmonellosis (typhoid and NTS disease) in suspected pediatric cases that present with undefined febrile disease.

AB - Inexpensive, easy-to-use, and highly sensitive diagnostic tests are currently unavailable for typhoid fever. To identify candidate serodiagnostic markers, we have probed microarrays displaying the full Salmonella enterica serovar Typhi (S. Typhi) proteome of 4,352 different proteins + lipopolysaccharides (LPSs), with sera from Nigerian pediatric typhoid and other febrile cases, Nigerian healthy controls, and healthy U.S. adults. Nigerian antibody profiles were broad (∼500 seropositive antigens) and mainly low level, with a small number of stronger "hits," whereas the profile in U.S. adults was < 1/5 as broad, consistent with endemic exposure in Nigeria. Nigerian profiles were largely unaffected by clinical diagnosis, although the response against t1477 (hemolysin E) consistently emerged as stronger in typhoid cases. The response to LPS was also a strong discriminator of healthy controls and typhoid, although LPS did not discriminate between typhoid and nontyphoidal Salmonella (NTS) disease. As a first step toward the development of a point-of-care diagnostic, t1477 and LPS were evaluated on immunostrips. Both provided good discrimination between healthy controls and typhoid/NTS disease. Such a test could provide a useful screen for salmonellosis (typhoid and NTS disease) in suspected pediatric cases that present with undefined febrile disease.

UR - http://www.scopus.com/inward/record.url?scp=84982782725&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84982782725&partnerID=8YFLogxK

U2 - 10.4269/ajtmh.15-0869

DO - 10.4269/ajtmh.15-0869

M3 - Article

VL - 95

SP - 431

EP - 439

JO - American Journal of Tropical Medicine and Hygiene

JF - American Journal of Tropical Medicine and Hygiene

SN - 0002-9637

IS - 2

ER -