Serine/Threonine Protein Phosphatase Is Required for Tobacco Mosaic Virus-Mediated Programmed Cell Death

David D Dunigan, John C. Madlener

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

A major gap in our understanding of host response to virus infection is how the molecular signals are passed within infected cells. Tobacco mosaic virus-mediated programmed cell death in genotype NN tobaccos was used to evaluate the hypothesis that these molecular signals are transduced via reversible-protein phosphorylation. Nicotiana tabacum L. (genotype NN) confers a hypersensitive response at the site of virus infection when incubated at a permissive temperature. Activation of serine/threonine protein phosphatase correlated with the temperature-dependent induction of the death program. The serine/threonine protein phosphatase inhibitor okadaic acid inhibited the onset and extent of the hypersensitive response in vivo. Biochemical analysis indicates that protein phosphatase type 1 is activated early in the death program. This is the first indication that serine/threonine protein phosphatase is required in an early event of the host response to virus infection.

Original languageEnglish (US)
Article number71105
Pages (from-to)460-466
Number of pages7
JournalVirology
Volume207
Issue number2
DOIs
StatePublished - Mar 10 1995

Fingerprint

Tobacco Mosaic Virus
Phosphoprotein Phosphatases
Virus Diseases
Cell Death
Tobacco
Genotype
Protein Phosphatase 1
Okadaic Acid
Temperature
Phosphorylation
Proteins

ASJC Scopus subject areas

  • Virology

Cite this

Serine/Threonine Protein Phosphatase Is Required for Tobacco Mosaic Virus-Mediated Programmed Cell Death. / Dunigan, David D; Madlener, John C.

In: Virology, Vol. 207, No. 2, 71105, 10.03.1995, p. 460-466.

Research output: Contribution to journalArticle

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