Serial diffusion tensor MRI after transient and permanent cerebral ischemia in nonhuman primates

Yutong Liu, Helen E. D'Arceuil, Susan Westmoreland, Julian He, Michael Duggan, R. Gilberto Gonzalez, Johnny Pryor, Alex J. De Crespigny

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

BACKGROUND AND PURPOSE - We measured the temporal evolution of the T2 and diffusion tensor imaging parameters after transient and permanent cerebral middle cerebral artery occlusion (MCAo) in macaques, and compared it to standard histological analysis at the study end point. METHODS - Stroke was created in adult male macaques by occluding a middle cerebral artery branch for 3 hours (transient MCAo, n=4 or permanent occlusion, n=3). Conventional MRI and diffusion tensor imaging scans were performed 0 (acute day), 1, 3, 7, 10, 17, and 30 days after MCAo. Animals were euthanized after the final scan and the brains removed for histological analysis. RESULTS - Apparent diffusion coefficient in the lesion was decreased acutely, fractional anisotropy was elevated, and T2 remained normal. Thereafter, apparent diffusion coefficient increased above normal, fractional anisotropy decreased to below normal, T2 increased to a maximum and then declined. Reperfusion at 3 hours accelerated these MRI changes. Only the fractional anisotropy value was significantly different between transient and permanent groups at 30 days. Final MRI-defined fractional lesion volumes were well correlated with corresponding histological lesion volumes. Permanent MCAO animals showed more severe histological damage than their transient MCAO counterparts, especially myelin damage and axonal swelling. CONCLUSIONS - Overall, the MRI evolution of stroke in macaques was closer to what has been observed in humans than in rodent models. This work supports the use of serial MRI in stroke studies in nonhuman primates.

Original languageEnglish (US)
Pages (from-to)138-145
Number of pages8
JournalStroke
Volume38
Issue number1
DOIs
StatePublished - Jan 1 2007

Fingerprint

Diffusion Magnetic Resonance Imaging
Middle Cerebral Artery Infarction
Transient Ischemic Attack
Anisotropy
Macaca
Primates
Diffusion Tensor Imaging
Stroke
Middle Cerebral Artery
Myelin Sheath
Reperfusion
Rodentia
Brain

Keywords

  • Brain imaging
  • Diffusion tensor imaging
  • Diffusion-weighted imaging
  • Experimental
  • Focal ischemia
  • Interventional neuroradiology
  • MRI
  • Magnetic resonance
  • Neuroradiology
  • Pathology ischemia
  • Stroke

ASJC Scopus subject areas

  • Clinical Neurology
  • Cardiology and Cardiovascular Medicine
  • Advanced and Specialized Nursing

Cite this

Liu, Y., D'Arceuil, H. E., Westmoreland, S., He, J., Duggan, M., Gonzalez, R. G., ... De Crespigny, A. J. (2007). Serial diffusion tensor MRI after transient and permanent cerebral ischemia in nonhuman primates. Stroke, 38(1), 138-145. https://doi.org/10.1161/01.STR.0000252127.07428.9c

Serial diffusion tensor MRI after transient and permanent cerebral ischemia in nonhuman primates. / Liu, Yutong; D'Arceuil, Helen E.; Westmoreland, Susan; He, Julian; Duggan, Michael; Gonzalez, R. Gilberto; Pryor, Johnny; De Crespigny, Alex J.

In: Stroke, Vol. 38, No. 1, 01.01.2007, p. 138-145.

Research output: Contribution to journalArticle

Liu, Y, D'Arceuil, HE, Westmoreland, S, He, J, Duggan, M, Gonzalez, RG, Pryor, J & De Crespigny, AJ 2007, 'Serial diffusion tensor MRI after transient and permanent cerebral ischemia in nonhuman primates', Stroke, vol. 38, no. 1, pp. 138-145. https://doi.org/10.1161/01.STR.0000252127.07428.9c
Liu, Yutong ; D'Arceuil, Helen E. ; Westmoreland, Susan ; He, Julian ; Duggan, Michael ; Gonzalez, R. Gilberto ; Pryor, Johnny ; De Crespigny, Alex J. / Serial diffusion tensor MRI after transient and permanent cerebral ischemia in nonhuman primates. In: Stroke. 2007 ; Vol. 38, No. 1. pp. 138-145.
@article{8270a6d36ebd4f41b0e4b3f68ebd15f5,
title = "Serial diffusion tensor MRI after transient and permanent cerebral ischemia in nonhuman primates",
abstract = "BACKGROUND AND PURPOSE - We measured the temporal evolution of the T2 and diffusion tensor imaging parameters after transient and permanent cerebral middle cerebral artery occlusion (MCAo) in macaques, and compared it to standard histological analysis at the study end point. METHODS - Stroke was created in adult male macaques by occluding a middle cerebral artery branch for 3 hours (transient MCAo, n=4 or permanent occlusion, n=3). Conventional MRI and diffusion tensor imaging scans were performed 0 (acute day), 1, 3, 7, 10, 17, and 30 days after MCAo. Animals were euthanized after the final scan and the brains removed for histological analysis. RESULTS - Apparent diffusion coefficient in the lesion was decreased acutely, fractional anisotropy was elevated, and T2 remained normal. Thereafter, apparent diffusion coefficient increased above normal, fractional anisotropy decreased to below normal, T2 increased to a maximum and then declined. Reperfusion at 3 hours accelerated these MRI changes. Only the fractional anisotropy value was significantly different between transient and permanent groups at 30 days. Final MRI-defined fractional lesion volumes were well correlated with corresponding histological lesion volumes. Permanent MCAO animals showed more severe histological damage than their transient MCAO counterparts, especially myelin damage and axonal swelling. CONCLUSIONS - Overall, the MRI evolution of stroke in macaques was closer to what has been observed in humans than in rodent models. This work supports the use of serial MRI in stroke studies in nonhuman primates.",
keywords = "Brain imaging, Diffusion tensor imaging, Diffusion-weighted imaging, Experimental, Focal ischemia, Interventional neuroradiology, MRI, Magnetic resonance, Neuroradiology, Pathology ischemia, Stroke",
author = "Yutong Liu and D'Arceuil, {Helen E.} and Susan Westmoreland and Julian He and Michael Duggan and Gonzalez, {R. Gilberto} and Johnny Pryor and {De Crespigny}, {Alex J.}",
year = "2007",
month = "1",
day = "1",
doi = "10.1161/01.STR.0000252127.07428.9c",
language = "English (US)",
volume = "38",
pages = "138--145",
journal = "Stroke; a journal of cerebral circulation",
issn = "0039-2499",
publisher = "Lippincott Williams and Wilkins",
number = "1",

}

TY - JOUR

T1 - Serial diffusion tensor MRI after transient and permanent cerebral ischemia in nonhuman primates

AU - Liu, Yutong

AU - D'Arceuil, Helen E.

AU - Westmoreland, Susan

AU - He, Julian

AU - Duggan, Michael

AU - Gonzalez, R. Gilberto

AU - Pryor, Johnny

AU - De Crespigny, Alex J.

PY - 2007/1/1

Y1 - 2007/1/1

N2 - BACKGROUND AND PURPOSE - We measured the temporal evolution of the T2 and diffusion tensor imaging parameters after transient and permanent cerebral middle cerebral artery occlusion (MCAo) in macaques, and compared it to standard histological analysis at the study end point. METHODS - Stroke was created in adult male macaques by occluding a middle cerebral artery branch for 3 hours (transient MCAo, n=4 or permanent occlusion, n=3). Conventional MRI and diffusion tensor imaging scans were performed 0 (acute day), 1, 3, 7, 10, 17, and 30 days after MCAo. Animals were euthanized after the final scan and the brains removed for histological analysis. RESULTS - Apparent diffusion coefficient in the lesion was decreased acutely, fractional anisotropy was elevated, and T2 remained normal. Thereafter, apparent diffusion coefficient increased above normal, fractional anisotropy decreased to below normal, T2 increased to a maximum and then declined. Reperfusion at 3 hours accelerated these MRI changes. Only the fractional anisotropy value was significantly different between transient and permanent groups at 30 days. Final MRI-defined fractional lesion volumes were well correlated with corresponding histological lesion volumes. Permanent MCAO animals showed more severe histological damage than their transient MCAO counterparts, especially myelin damage and axonal swelling. CONCLUSIONS - Overall, the MRI evolution of stroke in macaques was closer to what has been observed in humans than in rodent models. This work supports the use of serial MRI in stroke studies in nonhuman primates.

AB - BACKGROUND AND PURPOSE - We measured the temporal evolution of the T2 and diffusion tensor imaging parameters after transient and permanent cerebral middle cerebral artery occlusion (MCAo) in macaques, and compared it to standard histological analysis at the study end point. METHODS - Stroke was created in adult male macaques by occluding a middle cerebral artery branch for 3 hours (transient MCAo, n=4 or permanent occlusion, n=3). Conventional MRI and diffusion tensor imaging scans were performed 0 (acute day), 1, 3, 7, 10, 17, and 30 days after MCAo. Animals were euthanized after the final scan and the brains removed for histological analysis. RESULTS - Apparent diffusion coefficient in the lesion was decreased acutely, fractional anisotropy was elevated, and T2 remained normal. Thereafter, apparent diffusion coefficient increased above normal, fractional anisotropy decreased to below normal, T2 increased to a maximum and then declined. Reperfusion at 3 hours accelerated these MRI changes. Only the fractional anisotropy value was significantly different between transient and permanent groups at 30 days. Final MRI-defined fractional lesion volumes were well correlated with corresponding histological lesion volumes. Permanent MCAO animals showed more severe histological damage than their transient MCAO counterparts, especially myelin damage and axonal swelling. CONCLUSIONS - Overall, the MRI evolution of stroke in macaques was closer to what has been observed in humans than in rodent models. This work supports the use of serial MRI in stroke studies in nonhuman primates.

KW - Brain imaging

KW - Diffusion tensor imaging

KW - Diffusion-weighted imaging

KW - Experimental

KW - Focal ischemia

KW - Interventional neuroradiology

KW - MRI

KW - Magnetic resonance

KW - Neuroradiology

KW - Pathology ischemia

KW - Stroke

UR - http://www.scopus.com/inward/record.url?scp=33845959610&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33845959610&partnerID=8YFLogxK

U2 - 10.1161/01.STR.0000252127.07428.9c

DO - 10.1161/01.STR.0000252127.07428.9c

M3 - Article

VL - 38

SP - 138

EP - 145

JO - Stroke; a journal of cerebral circulation

JF - Stroke; a journal of cerebral circulation

SN - 0039-2499

IS - 1

ER -