Sera from patients with age-related cataract have antibody to a hepatoma derived growth factor-like protein

Dhirendra P Singh, T. Kikuchi, T. Sueno

Research output: Contribution to journalArticle

Abstract

Purpose: The goal of this study is to find cause(s) of the age-related cataract (ARC). Previously, we have reported that auto-antibodies (Abs) play an important role in ARC formation. We identified 48 kDa protein from human lens (HL) to which auto-Ab is directed. Methods: We screened a cDNA library with human sera from ARC patients, constructed from mRNAs of 100 human capsulotomy specimens containing lens epithelial cells {LECs) and determined a sequence of cDNA. The protein was expressed in an eukaryotic expression system. The Abs were raised and tissue specificity of the protein examined by immunohistochemistry. Results: We isolated a clone named HL48 kDa protein was found a to have high sequence homology to the hepatoma-derived growth factor. The HL48 kDa protein stimulated growth of mouse LECs and was neutralized by the Ab. Immunocytochemistry with Ab probes and Northern blot analysis with cDNA probes showed that the protein was predominantly expressed in LECs, spleen cells, and lymphocytes. The HL48 kDa protein was highly modified in the human capsulotomy specimens but was not modified in cultured LECs. The Abs to this protein were found in most sera from cataract patients. Conclusions The HL48 kDa protein affects growth and differentiation of LECs. The auto-Abs suppress these effects and thus may contribute to formation ARC.

Original languageEnglish (US)
JournalInvestigative Ophthalmology and Visual Science
Volume38
Issue number4
StatePublished - Dec 1 1997

Fingerprint

Cataract
Antibodies
Lenses
Serum
Epithelial Cells
Proteins
Complementary DNA
Immunohistochemistry
Organ Specificity
Crystallins
hepatoma-derived growth factor
Growth
Sequence Homology
Gene Library
Northern Blotting
Spleen
Clone Cells
Lymphocytes
Messenger RNA

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

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title = "Sera from patients with age-related cataract have antibody to a hepatoma derived growth factor-like protein",
abstract = "Purpose: The goal of this study is to find cause(s) of the age-related cataract (ARC). Previously, we have reported that auto-antibodies (Abs) play an important role in ARC formation. We identified 48 kDa protein from human lens (HL) to which auto-Ab is directed. Methods: We screened a cDNA library with human sera from ARC patients, constructed from mRNAs of 100 human capsulotomy specimens containing lens epithelial cells {LECs) and determined a sequence of cDNA. The protein was expressed in an eukaryotic expression system. The Abs were raised and tissue specificity of the protein examined by immunohistochemistry. Results: We isolated a clone named HL48 kDa protein was found a to have high sequence homology to the hepatoma-derived growth factor. The HL48 kDa protein stimulated growth of mouse LECs and was neutralized by the Ab. Immunocytochemistry with Ab probes and Northern blot analysis with cDNA probes showed that the protein was predominantly expressed in LECs, spleen cells, and lymphocytes. The HL48 kDa protein was highly modified in the human capsulotomy specimens but was not modified in cultured LECs. The Abs to this protein were found in most sera from cataract patients. Conclusions The HL48 kDa protein affects growth and differentiation of LECs. The auto-Abs suppress these effects and thus may contribute to formation ARC.",
author = "Singh, {Dhirendra P} and T. Kikuchi and T. Sueno",
year = "1997",
month = "12",
day = "1",
language = "English (US)",
volume = "38",
journal = "Investigative Ophthalmology and Visual Science",
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TY - JOUR

T1 - Sera from patients with age-related cataract have antibody to a hepatoma derived growth factor-like protein

AU - Singh, Dhirendra P

AU - Kikuchi, T.

AU - Sueno, T.

PY - 1997/12/1

Y1 - 1997/12/1

N2 - Purpose: The goal of this study is to find cause(s) of the age-related cataract (ARC). Previously, we have reported that auto-antibodies (Abs) play an important role in ARC formation. We identified 48 kDa protein from human lens (HL) to which auto-Ab is directed. Methods: We screened a cDNA library with human sera from ARC patients, constructed from mRNAs of 100 human capsulotomy specimens containing lens epithelial cells {LECs) and determined a sequence of cDNA. The protein was expressed in an eukaryotic expression system. The Abs were raised and tissue specificity of the protein examined by immunohistochemistry. Results: We isolated a clone named HL48 kDa protein was found a to have high sequence homology to the hepatoma-derived growth factor. The HL48 kDa protein stimulated growth of mouse LECs and was neutralized by the Ab. Immunocytochemistry with Ab probes and Northern blot analysis with cDNA probes showed that the protein was predominantly expressed in LECs, spleen cells, and lymphocytes. The HL48 kDa protein was highly modified in the human capsulotomy specimens but was not modified in cultured LECs. The Abs to this protein were found in most sera from cataract patients. Conclusions The HL48 kDa protein affects growth and differentiation of LECs. The auto-Abs suppress these effects and thus may contribute to formation ARC.

AB - Purpose: The goal of this study is to find cause(s) of the age-related cataract (ARC). Previously, we have reported that auto-antibodies (Abs) play an important role in ARC formation. We identified 48 kDa protein from human lens (HL) to which auto-Ab is directed. Methods: We screened a cDNA library with human sera from ARC patients, constructed from mRNAs of 100 human capsulotomy specimens containing lens epithelial cells {LECs) and determined a sequence of cDNA. The protein was expressed in an eukaryotic expression system. The Abs were raised and tissue specificity of the protein examined by immunohistochemistry. Results: We isolated a clone named HL48 kDa protein was found a to have high sequence homology to the hepatoma-derived growth factor. The HL48 kDa protein stimulated growth of mouse LECs and was neutralized by the Ab. Immunocytochemistry with Ab probes and Northern blot analysis with cDNA probes showed that the protein was predominantly expressed in LECs, spleen cells, and lymphocytes. The HL48 kDa protein was highly modified in the human capsulotomy specimens but was not modified in cultured LECs. The Abs to this protein were found in most sera from cataract patients. Conclusions The HL48 kDa protein affects growth and differentiation of LECs. The auto-Abs suppress these effects and thus may contribute to formation ARC.

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