Sequence-variant repeats of MUC1 show higher conformational flexibility, are less densely O-glycosylated and induce differential B lymphocyte responses

Silvia von Mensdorff-Pouilly, Leo Kinarsky, Katja Engelmann, Stephan E. Baldus, René H. Verheijen, Michael A Hollingsworth, Vladimir Pisarev, Simon Sherman, Franz Georg Hanisch

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The human epithelial cancer mucin MUC1 is able to break tolerance and to induce humoral immune responses in healthy subjects and in cancer patients. We recently showed that clusters of sequence-variant repeats are interspersed in the repeat domain of MUC1 at high frequency, which should contribute to the structural and immunological features of the mucin. Here we elucidated the potential effects exerted by sequence-variant repeats on their O-glycosylation. Evidence from in vitro glycosylation with polypeptide N-acetylgalactosaminyltransferases GalNAc-T1 and GalNAc-T2 in concert with mass spectrometric analyses of in vivo glycosylated MUC1 probes from transiently transfected HEK293 cells indicated reduced glycosylation densities of repeats with three concerted replacements: AHGVTSAPESRPAPGSTAPA. The Pro to Ala replacement in STAPA exerts not only proximal effects on the ppGalNAc-T2 preferred site at -3 and -4, but also more distant effects on the ppGalNAc-T1 preferred site at -15 (TSAPESRPAPGSTAPA We also examined the conformational changes of MUC1 glycopeptides induced by the concerted DT to ES replacements and revealed a higher conformational flexibility of ES/P peptides compared to DT/P peptides. Differences in conformational flexibilities and in O-glycosylation densities could underlie the observed differential humoral responses in humans. We were able to show that the natural immunoglobulin G (IgG) responses to the repeat domain of MUC1 in sera from nonmalignant control subjects are preferentially directed to variant repeat clusters. In contrast, the IgG response in patients with adenocarcinoma shifted to higher frequencies of preferential DTR peptide binding.

Original languageEnglish (US)
Pages (from-to)735-746
Number of pages12
JournalGlycobiology
Volume15
Issue number8
DOIs
StatePublished - Aug 1 2005

Fingerprint

Glycosylation
Lymphocytes
B-Lymphocytes
Mucins
Immunoglobulin G
Glycopeptides
HEK293 Cells
Humoral Immunity
Neoplasms
Healthy Volunteers
Adenocarcinoma
Peptides
Serum
polypeptide N-acetylgalactosaminyltransferase
peptide P

Keywords

  • B cell response
  • Cancer
  • MUC1
  • O-glycosylation
  • Peptide conformation

ASJC Scopus subject areas

  • Biochemistry

Cite this

Sequence-variant repeats of MUC1 show higher conformational flexibility, are less densely O-glycosylated and induce differential B lymphocyte responses. / von Mensdorff-Pouilly, Silvia; Kinarsky, Leo; Engelmann, Katja; Baldus, Stephan E.; Verheijen, René H.; Hollingsworth, Michael A; Pisarev, Vladimir; Sherman, Simon; Hanisch, Franz Georg.

In: Glycobiology, Vol. 15, No. 8, 01.08.2005, p. 735-746.

Research output: Contribution to journalArticle

von Mensdorff-Pouilly, Silvia ; Kinarsky, Leo ; Engelmann, Katja ; Baldus, Stephan E. ; Verheijen, René H. ; Hollingsworth, Michael A ; Pisarev, Vladimir ; Sherman, Simon ; Hanisch, Franz Georg. / Sequence-variant repeats of MUC1 show higher conformational flexibility, are less densely O-glycosylated and induce differential B lymphocyte responses. In: Glycobiology. 2005 ; Vol. 15, No. 8. pp. 735-746.
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T1 - Sequence-variant repeats of MUC1 show higher conformational flexibility, are less densely O-glycosylated and induce differential B lymphocyte responses

AU - von Mensdorff-Pouilly, Silvia

AU - Kinarsky, Leo

AU - Engelmann, Katja

AU - Baldus, Stephan E.

AU - Verheijen, René H.

AU - Hollingsworth, Michael A

AU - Pisarev, Vladimir

AU - Sherman, Simon

AU - Hanisch, Franz Georg

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N2 - The human epithelial cancer mucin MUC1 is able to break tolerance and to induce humoral immune responses in healthy subjects and in cancer patients. We recently showed that clusters of sequence-variant repeats are interspersed in the repeat domain of MUC1 at high frequency, which should contribute to the structural and immunological features of the mucin. Here we elucidated the potential effects exerted by sequence-variant repeats on their O-glycosylation. Evidence from in vitro glycosylation with polypeptide N-acetylgalactosaminyltransferases GalNAc-T1 and GalNAc-T2 in concert with mass spectrometric analyses of in vivo glycosylated MUC1 probes from transiently transfected HEK293 cells indicated reduced glycosylation densities of repeats with three concerted replacements: AHGVTSAPESRPAPGSTAPA. The Pro to Ala replacement in STAPA exerts not only proximal effects on the ppGalNAc-T2 preferred site at -3 and -4, but also more distant effects on the ppGalNAc-T1 preferred site at -15 (TSAPESRPAPGSTAPA We also examined the conformational changes of MUC1 glycopeptides induced by the concerted DT to ES replacements and revealed a higher conformational flexibility of ES/P peptides compared to DT/P peptides. Differences in conformational flexibilities and in O-glycosylation densities could underlie the observed differential humoral responses in humans. We were able to show that the natural immunoglobulin G (IgG) responses to the repeat domain of MUC1 in sera from nonmalignant control subjects are preferentially directed to variant repeat clusters. In contrast, the IgG response in patients with adenocarcinoma shifted to higher frequencies of preferential DTR peptide binding.

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KW - B cell response

KW - Cancer

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KW - O-glycosylation

KW - Peptide conformation

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