Sequence-specific, electronic detection of oligonucleotides in blood, soil, and foodstuffs with the reagentless, reusable E-DNA sensor

Arica A. Lubin, Rebecca Y. Lai, Brian R. Baker, Alan J. Heeger, Kevin W. Plaxco

Research output: Contribution to journalArticle

165 Citations (Scopus)

Abstract

The ability to detect specific oligonucleotides in complex, contaminant-ridden samples, without the use of exogenous reagents and using a reusable, fully electronic platform could revolutionize the detection of pathogens in the clinic and in the field. Here, we characterize a label-free, electronic sensor, termed E-DNA, for its ability to simultaneously meet these challenging demands. We find that because signal generation is coupled to a hybridization-linked conformational change, rather than to only adsorption to the sensor surface, E-DNA is selective enough to detect oligonucleotides in complex, multicomponent samples, such as blood serum and soil. Moreover, E-DNA signaling is monotonically related to target complementarity, allowing the sensor to discriminate between mismatched targets: we readily detect the complementary 17-base target against a 50 000-fold excess of genomic DNA, can distinguish a three-base mismatch from perfect target directly in blood serum, and under ideal conditions, observe statistically significant differences between single-base mismatches. Finally, because the sensing components are linked to the electrode surface, E-DNA is reusable: a 30-s room temperature wash recovers >99% of the sensor signal. This work further supports the utility of E-DNA as a rapid, specific, and convenient method for the detection of DNA and RNA sequences.

Original languageEnglish (US)
Pages (from-to)5671-5677
Number of pages7
JournalAnalytical chemistry
Volume78
Issue number16
DOIs
StatePublished - Aug 15 2006

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Oligonucleotides
Blood
Soils
DNA
Sensors
Pathogens
Labels
RNA
Impurities
Adsorption
Electrodes

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Sequence-specific, electronic detection of oligonucleotides in blood, soil, and foodstuffs with the reagentless, reusable E-DNA sensor. / Lubin, Arica A.; Lai, Rebecca Y.; Baker, Brian R.; Heeger, Alan J.; Plaxco, Kevin W.

In: Analytical chemistry, Vol. 78, No. 16, 15.08.2006, p. 5671-5677.

Research output: Contribution to journalArticle

Lubin, Arica A. ; Lai, Rebecca Y. ; Baker, Brian R. ; Heeger, Alan J. ; Plaxco, Kevin W. / Sequence-specific, electronic detection of oligonucleotides in blood, soil, and foodstuffs with the reagentless, reusable E-DNA sensor. In: Analytical chemistry. 2006 ; Vol. 78, No. 16. pp. 5671-5677.
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