Sequence and annotation of the 314-kb MT325 and the 321-kb FR483 viruses that infect Chlorella Pbi

Lisa A. Fitzgerald, Michael V. Graves, Xiao Li, Tamara Feldblyum, James Hartigan, James L Van Etten

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Viruses MT325 and FR483, members of the family Phycodnaviridae, genus Chlorovirus, infect the fresh water, unicellular, eukaryotic, chlorella-like green alga, Chlorella Pbi. The 314,335-bp genome of MT325 and the 321,240-bp genome of FR483 are the first viruses that infect Chlorella Pbi to have their genomes sequenced and annotated. Furthermore, these genomes are the two smallest chlorella virus genomes sequenced to date, MT325 has 331 putative protein-encoding and 10 tRNA-encoding genes and FR483 has 335 putative protein-encoding and 9 tRNA-encoding genes. The protein-encoding genes are almost evenly distributed on both strands, and intergenic space is minimal. Approximately 40% of the viral gene products resemble entries in public databases, including some that are the first of their kind to be detected in a virus. For example, these unique gene products include an aquaglyceroporin in MT325, a potassium ion transporter protein and an alkyl sulfatase in FR483, and a dTDP-glucose pyrophosphorylase in both viruses. Comparison of MT325 and FR483 protein-encoding genes with the prototype chlorella virus PBCV-1 indicates that approximately 82% of the genes are present in all three viruses.

Original languageEnglish (US)
Pages (from-to)459-471
Number of pages13
JournalVirology
Volume358
Issue number2
DOIs
StatePublished - Feb 20 2007

Fingerprint

Chlorella
Viruses
Genome
Transfer RNA
Proteins
Genes
Phycodnaviridae
Aquaglyceroporins
Sulfatases
Chlorophyta
Viral Proteins
Fresh Water
Potassium
Databases
Ions

Keywords

  • Chlorella viruses
  • Genome sequence
  • Phycodnaviridae
  • Virus FR483
  • Virus MT325

ASJC Scopus subject areas

  • Virology

Cite this

Sequence and annotation of the 314-kb MT325 and the 321-kb FR483 viruses that infect Chlorella Pbi. / Fitzgerald, Lisa A.; Graves, Michael V.; Li, Xiao; Feldblyum, Tamara; Hartigan, James; Van Etten, James L.

In: Virology, Vol. 358, No. 2, 20.02.2007, p. 459-471.

Research output: Contribution to journalArticle

Fitzgerald, Lisa A. ; Graves, Michael V. ; Li, Xiao ; Feldblyum, Tamara ; Hartigan, James ; Van Etten, James L. / Sequence and annotation of the 314-kb MT325 and the 321-kb FR483 viruses that infect Chlorella Pbi. In: Virology. 2007 ; Vol. 358, No. 2. pp. 459-471.
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