Semiconservative synthesis of single-stranded RNA by bacteriophage phi6 RNA polymerase

J. L. van Etten, D. E. Burbank, D. A. Cuppels, L. C. Lane, A. K. Vidaver

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

The RNA polymerase in the nucleocapsid of Pseudomonas phaseolicola bacteriophage phi6 transcribed large, medium, and small single-stranded RNA from the viral double-stranded RNA genome by a semiconservative (displacement) mechanism. Approximately 23%, 63%, and 65% of the nucleocapsid particles in the assay mixture synthesized at least one round of large, medium, and small single-stranded RNA molecules, respectively. Some of these particles reinitiated synthesis such that an average of 1.5 large, 33 medium, and 24 small single-stranded RNAs were synthesized from each double-stranded RNA.

Original languageEnglish (US)
Pages (from-to)769-773
Number of pages5
JournalJournal of virology
Volume33
Issue number2
StatePublished - Apr 23 1980

Fingerprint

RNA Phages
Nucleocapsid
Double-Stranded RNA
DNA-Directed RNA Polymerases
DNA-directed RNA polymerase
bacteriophages
nucleocapsid
Pseudomonas Phages
double-stranded RNA
RNA
synthesis
Viral RNA
Genome
Pseudomonas
genome
assays

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

van Etten, J. L., Burbank, D. E., Cuppels, D. A., Lane, L. C., & Vidaver, A. K. (1980). Semiconservative synthesis of single-stranded RNA by bacteriophage phi6 RNA polymerase. Journal of virology, 33(2), 769-773.

Semiconservative synthesis of single-stranded RNA by bacteriophage phi6 RNA polymerase. / van Etten, J. L.; Burbank, D. E.; Cuppels, D. A.; Lane, L. C.; Vidaver, A. K.

In: Journal of virology, Vol. 33, No. 2, 23.04.1980, p. 769-773.

Research output: Contribution to journalArticle

van Etten, JL, Burbank, DE, Cuppels, DA, Lane, LC & Vidaver, AK 1980, 'Semiconservative synthesis of single-stranded RNA by bacteriophage phi6 RNA polymerase', Journal of virology, vol. 33, no. 2, pp. 769-773.
van Etten, J. L. ; Burbank, D. E. ; Cuppels, D. A. ; Lane, L. C. ; Vidaver, A. K. / Semiconservative synthesis of single-stranded RNA by bacteriophage phi6 RNA polymerase. In: Journal of virology. 1980 ; Vol. 33, No. 2. pp. 769-773.
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