Semiconservative synthesis of single-stranded RNA by bacteriophage phi6 RNA polymerase

J. L. van Etten, D. E. Burbank, D. A. Cuppels, L. C. Lane, A. K. Vidaver

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Abstract

The RNA polymerase in the nucleocapsid of Pseudomonas phaseolicola bacteriophage phi6 transcribed large, medium, and small single-stranded RNA from the viral double-stranded RNA genome by a semiconservative (displacement) mechanism. Approximately 23%, 63%, and 65% of the nucleocapsid particles in the assay mixture synthesized at least one round of large, medium, and small single-stranded RNA molecules, respectively. Some of these particles reinitiated synthesis such that an average of 1.5 large, 33 medium, and 24 small single-stranded RNAs were synthesized from each double-stranded RNA.

Original languageEnglish (US)
Pages (from-to)769-773
Number of pages5
JournalJournal of virology
Volume33
Issue number2
Publication statusPublished - Apr 23 1980

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van Etten, J. L., Burbank, D. E., Cuppels, D. A., Lane, L. C., & Vidaver, A. K. (1980). Semiconservative synthesis of single-stranded RNA by bacteriophage phi6 RNA polymerase. Journal of virology, 33(2), 769-773.