Selective recognition of DNA antigenic determinants by murine monoclonal anti-DNA antibodies

D. P. Wu, G. S. Gilkeson, James Olen Armitage, C. F. Reich, D. S. Pisetsky

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

To assess the immune recognition of DNA in systemic lupus erythematosus, the antigenic specificity of monoclonal anti-DNA antibodies from autoimmune MRL-lpr/lpr mice was investigated. Determinant specificity was assessed by ELISA in terms of binding to a panel of ssDNA antigens including calf thymus, human placenta, Escherichia coli, Clostridium perfringens, Micrococcus lysodeikticus, salmon testes, chicken blood and murine DNA. Among the monoclonal antibodies, a variety of binding patterns was observed, although for all antibodies tested murine DNA was among the most reactive antigens. Binding to other DNAs varied markedly, with some antibodies showing only low reactivity to certain antigens in the test panel. Similar results were obtained with sera of individual MRL-lpr/lpr mice. These results suggest that anti-DNA antibodies bind specific antigenic determinants variably expressed by DNAs of various species. Furthermore, the preferential binding to mouse DNA by some MRL-lpr/lpr antibodies may suggest a role of self-DNA in the in vivo selection of anti-DNA antibodies for expression.

Original languageEnglish (US)
Pages (from-to)33-37
Number of pages5
JournalClinical and Experimental Immunology
Volume82
Issue number1
StatePublished - Jan 1 1990

Fingerprint

Antinuclear Antibodies
Epitopes
Monoclonal Antibodies
DNA
Antigens
Antibodies
Micrococcus
Clostridium perfringens
Salmon
Systemic Lupus Erythematosus
Thymus Gland
Placenta
Testis
Chickens
Enzyme-Linked Immunosorbent Assay
Escherichia coli
Serum

Keywords

  • DNA antigenicity
  • anti-DNA antibodies
  • monoclonal antibodies
  • murine models of autoimmunity
  • systemic lupus erythematosus

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Selective recognition of DNA antigenic determinants by murine monoclonal anti-DNA antibodies. / Wu, D. P.; Gilkeson, G. S.; Armitage, James Olen; Reich, C. F.; Pisetsky, D. S.

In: Clinical and Experimental Immunology, Vol. 82, No. 1, 01.01.1990, p. 33-37.

Research output: Contribution to journalArticle

Wu, D. P. ; Gilkeson, G. S. ; Armitage, James Olen ; Reich, C. F. ; Pisetsky, D. S. / Selective recognition of DNA antigenic determinants by murine monoclonal anti-DNA antibodies. In: Clinical and Experimental Immunology. 1990 ; Vol. 82, No. 1. pp. 33-37.
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