Selective primary hepatocyte adhesion on polyelectrolyte multilayer

Template for patterned cell co-culture

Srivatsan S Kidambi, Ilsoon Lee, Christina Chan

Research output: Contribution to conferencePaper

1 Citation (Scopus)

Abstract

The successful attachment and spreading of primary hepatocytes on polyelectrolyte multilayer (PEM) film without the use of adhesive proteins such as collagen or fibronectin and patterns of co-cultures was described. It was demonstrated that patterns of primary hepatocytes and co-cultures can be formed using layer-by-layer deposition of ionic polymers. The synthetic polymers namely poly(diallyldimethulammoniumchloride) (PDAC) and sulfonated poly(styrene) (SPS) as the polycation and polyanion, respectively, were used to build the multilayers. SPS patterns were formed on PEM surfaces either by microcontact printing SPS onto PDAC surfaces of vice-versa. Co-culture patterns of fibroblast and primary hepatocytes were obtained on synthetic PEM surfaces.

Original languageEnglish (US)
Pages8351-8355
Number of pages5
StatePublished - Dec 1 2004
Event2004 AIChE Annual Meeting - Austin, TX, United States
Duration: Nov 7 2004Nov 12 2004

Conference

Conference2004 AIChE Annual Meeting
CountryUnited States
CityAustin, TX
Period11/7/0411/12/04

Fingerprint

Polyelectrolytes
Styrene
Multilayers
Adhesion
Multilayer films
Polymers
Fibroblasts
Cell culture
Collagen
Printing
Adhesives
Proteins
Hepatocytes

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Kidambi, S. S., Lee, I., & Chan, C. (2004). Selective primary hepatocyte adhesion on polyelectrolyte multilayer: Template for patterned cell co-culture. 8351-8355. Paper presented at 2004 AIChE Annual Meeting, Austin, TX, United States.

Selective primary hepatocyte adhesion on polyelectrolyte multilayer : Template for patterned cell co-culture. / Kidambi, Srivatsan S; Lee, Ilsoon; Chan, Christina.

2004. 8351-8355 Paper presented at 2004 AIChE Annual Meeting, Austin, TX, United States.

Research output: Contribution to conferencePaper

Kidambi, SS, Lee, I & Chan, C 2004, 'Selective primary hepatocyte adhesion on polyelectrolyte multilayer: Template for patterned cell co-culture' Paper presented at 2004 AIChE Annual Meeting, Austin, TX, United States, 11/7/04 - 11/12/04, pp. 8351-8355.
Kidambi SS, Lee I, Chan C. Selective primary hepatocyte adhesion on polyelectrolyte multilayer: Template for patterned cell co-culture. 2004. Paper presented at 2004 AIChE Annual Meeting, Austin, TX, United States.
Kidambi, Srivatsan S ; Lee, Ilsoon ; Chan, Christina. / Selective primary hepatocyte adhesion on polyelectrolyte multilayer : Template for patterned cell co-culture. Paper presented at 2004 AIChE Annual Meeting, Austin, TX, United States.5 p.
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