Selective Oma1 protease-mediated proteolysis of Cox1 subunit of cytochrome oxidase in assembly mutants

Oleh Khalimonchuk, Mi Young Jeong, Talina Watts, Elliott Ferris, Dennis R. Winge

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Stalled biogenesis of the mitochondrial cytochrome c oxidase (CcO) complex results in degradation of subunits containing redox cofactors. The conserved Oma1 metalloproteinase mediates facile Cox1 degradation in cells lacking the Coa2 assembly factor, but not in a series of other mutants stalled in CcO maturation. Oma1 is activated in coa2Δ cells, but the selective Cox1 degradation does not arise merely from its activation. Oma1 is also active in cells with dysfunctional mitochondria and cox11Δ cells impaired in CcO maturation, but this activation does not result in Oma1-mediated Cox1 degradation. The facile and selective degradation of Cox1 in coa2Δ cells, relative to other CcO assembly mutants, is likely due to impaired hemylation and subsequent misfolding of the subunit. Specific Cox1 proteolysis in coa2Δ cells arises from a combination of Oma1 activation and a susceptible conformation of Cox1.

Original languageEnglish (US)
Pages (from-to)7289-7300
Number of pages12
JournalJournal of Biological Chemistry
Volume287
Issue number10
DOIs
StatePublished - Mar 2 2012
Externally publishedYes

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Proteolysis
Electron Transport Complex IV
Peptide Hydrolases
Degradation
Chemical activation
Mitochondria
Metalloproteases
Organelle Biogenesis
Conformations
Oxidation-Reduction

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Selective Oma1 protease-mediated proteolysis of Cox1 subunit of cytochrome oxidase in assembly mutants. / Khalimonchuk, Oleh; Jeong, Mi Young; Watts, Talina; Ferris, Elliott; Winge, Dennis R.

In: Journal of Biological Chemistry, Vol. 287, No. 10, 02.03.2012, p. 7289-7300.

Research output: Contribution to journalArticle

Khalimonchuk, Oleh ; Jeong, Mi Young ; Watts, Talina ; Ferris, Elliott ; Winge, Dennis R. / Selective Oma1 protease-mediated proteolysis of Cox1 subunit of cytochrome oxidase in assembly mutants. In: Journal of Biological Chemistry. 2012 ; Vol. 287, No. 10. pp. 7289-7300.
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