Selective lipoxygenase inhibitor reduces bile acid-induced gastric mucosal injury

Theodore R. Sullivan, Juan A. Cordero, David W. Mercer, Wallace P. Ritchie, Daniel T. Dempsey

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Leukotriene receptor blockade attenuates topical bile acid-induced gastric mucosal injury, suggesting that peptidyl-leukotrienes may be mediators of this injury. The purpose of this study was to test the hypothesis that a selective 5-lipoxygenase inhibitor protects against bile acid-induced gastric epithelial injury in the rat. Prior to injury with 10 and 20 mM acidified taurocholate (pH 1.2), rat stomachs were pretreated with either vehicle or WY50295K (selective 5-lipoxygenase inhibitor, 20 mg/kg). Injury was assessed by measuring net transmucosal hydrogen ion flux, luminal appearance of DNA, and gross mucosal injury. Topical 5-lipoxygenase inhibitor significantly reduced luminal H+ ion loss, surface epithelial cell loss (as measured by luminal accumulation of DNA), and gross mucosal injury in bile acid-injured stomachs compared to controls. This study lends further support to the hypothesis that leukotrienes may be mediators of bile acid-induced gastric mucosal injury.

Original languageEnglish (US)
Pages (from-to)568-571
Number of pages4
JournalJournal of Surgical Research
Volume53
Issue number6
DOIs
StatePublished - Dec 1992

Fingerprint

Lipoxygenase Inhibitors
Bile Acids and Salts
Stomach
Wounds and Injuries
Leukotrienes
Leukotriene Receptors
Taurocholic Acid
DNA
Protons
Epithelial Cells
Ions

ASJC Scopus subject areas

  • Surgery

Cite this

Selective lipoxygenase inhibitor reduces bile acid-induced gastric mucosal injury. / Sullivan, Theodore R.; Cordero, Juan A.; Mercer, David W.; Ritchie, Wallace P.; Dempsey, Daniel T.

In: Journal of Surgical Research, Vol. 53, No. 6, 12.1992, p. 568-571.

Research output: Contribution to journalArticle

Sullivan, Theodore R. ; Cordero, Juan A. ; Mercer, David W. ; Ritchie, Wallace P. ; Dempsey, Daniel T. / Selective lipoxygenase inhibitor reduces bile acid-induced gastric mucosal injury. In: Journal of Surgical Research. 1992 ; Vol. 53, No. 6. pp. 568-571.
@article{df07e9e082ac458ea3f52ef61e2dd0df,
title = "Selective lipoxygenase inhibitor reduces bile acid-induced gastric mucosal injury",
abstract = "Leukotriene receptor blockade attenuates topical bile acid-induced gastric mucosal injury, suggesting that peptidyl-leukotrienes may be mediators of this injury. The purpose of this study was to test the hypothesis that a selective 5-lipoxygenase inhibitor protects against bile acid-induced gastric epithelial injury in the rat. Prior to injury with 10 and 20 mM acidified taurocholate (pH 1.2), rat stomachs were pretreated with either vehicle or WY50295K (selective 5-lipoxygenase inhibitor, 20 mg/kg). Injury was assessed by measuring net transmucosal hydrogen ion flux, luminal appearance of DNA, and gross mucosal injury. Topical 5-lipoxygenase inhibitor significantly reduced luminal H+ ion loss, surface epithelial cell loss (as measured by luminal accumulation of DNA), and gross mucosal injury in bile acid-injured stomachs compared to controls. This study lends further support to the hypothesis that leukotrienes may be mediators of bile acid-induced gastric mucosal injury.",
author = "Sullivan, {Theodore R.} and Cordero, {Juan A.} and Mercer, {David W.} and Ritchie, {Wallace P.} and Dempsey, {Daniel T.}",
year = "1992",
month = "12",
doi = "10.1016/0022-4804(92)90256-Y",
language = "English (US)",
volume = "53",
pages = "568--571",
journal = "Journal of Surgical Research",
issn = "0022-4804",
publisher = "Academic Press Inc.",
number = "6",

}

TY - JOUR

T1 - Selective lipoxygenase inhibitor reduces bile acid-induced gastric mucosal injury

AU - Sullivan, Theodore R.

AU - Cordero, Juan A.

AU - Mercer, David W.

AU - Ritchie, Wallace P.

AU - Dempsey, Daniel T.

PY - 1992/12

Y1 - 1992/12

N2 - Leukotriene receptor blockade attenuates topical bile acid-induced gastric mucosal injury, suggesting that peptidyl-leukotrienes may be mediators of this injury. The purpose of this study was to test the hypothesis that a selective 5-lipoxygenase inhibitor protects against bile acid-induced gastric epithelial injury in the rat. Prior to injury with 10 and 20 mM acidified taurocholate (pH 1.2), rat stomachs were pretreated with either vehicle or WY50295K (selective 5-lipoxygenase inhibitor, 20 mg/kg). Injury was assessed by measuring net transmucosal hydrogen ion flux, luminal appearance of DNA, and gross mucosal injury. Topical 5-lipoxygenase inhibitor significantly reduced luminal H+ ion loss, surface epithelial cell loss (as measured by luminal accumulation of DNA), and gross mucosal injury in bile acid-injured stomachs compared to controls. This study lends further support to the hypothesis that leukotrienes may be mediators of bile acid-induced gastric mucosal injury.

AB - Leukotriene receptor blockade attenuates topical bile acid-induced gastric mucosal injury, suggesting that peptidyl-leukotrienes may be mediators of this injury. The purpose of this study was to test the hypothesis that a selective 5-lipoxygenase inhibitor protects against bile acid-induced gastric epithelial injury in the rat. Prior to injury with 10 and 20 mM acidified taurocholate (pH 1.2), rat stomachs were pretreated with either vehicle or WY50295K (selective 5-lipoxygenase inhibitor, 20 mg/kg). Injury was assessed by measuring net transmucosal hydrogen ion flux, luminal appearance of DNA, and gross mucosal injury. Topical 5-lipoxygenase inhibitor significantly reduced luminal H+ ion loss, surface epithelial cell loss (as measured by luminal accumulation of DNA), and gross mucosal injury in bile acid-injured stomachs compared to controls. This study lends further support to the hypothesis that leukotrienes may be mediators of bile acid-induced gastric mucosal injury.

UR - http://www.scopus.com/inward/record.url?scp=0027052501&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027052501&partnerID=8YFLogxK

U2 - 10.1016/0022-4804(92)90256-Y

DO - 10.1016/0022-4804(92)90256-Y

M3 - Article

C2 - 1494289

AN - SCOPUS:0027052501

VL - 53

SP - 568

EP - 571

JO - Journal of Surgical Research

JF - Journal of Surgical Research

SN - 0022-4804

IS - 6

ER -