Selective laser sintered Ca-P/PHBV nanocomposite scaffolds with sustained release of rhBMP-2 for bone tissue engineering

Bin Duan, William W. Lu, Min Wang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Ca-P/PHBV nanocomposite scaffolds for bone tissue engineering were fabricated via selective laser sintering. The surface modification of Ca-P/PHBV scaffolds was conducted firstly by physical entrapment of gelatin. Heparin was then immobilized on gelatin-modified scaffolds through covalent conjugation. Human umbilical cord derived mesenchymal stem cells (hUC-MSCs) were seeded onto the scaffolds. Compared to non-modified scaffolds, heparin-immobilized scaffolds exhibited higher cell proliferation at the early stage of cell culture. hUC-MSCs became confluent after 21 day culture on scaffolds and covered the whole scaffold surface, strongly adhering to the scaffolds. Recombinant human bone morphogenetic protein (rhBMP)-2 was loaded onto scaffolds with or without surface modification and its in vitro release behavior was studied. An initial burst release of rhBMP-2 was observed for both types of scaffolds. However, the immobilization of heparin on the surface of Ca-P/PHBV scaffolds not only provided a means to protect the rhBMP-2 but also improved its sustained release. Surface modified scaffolds loaded with rhBMP-2 promoted significantly higher ALP activity of hUC-MSCs than the scaffolds with simple adsorption of rhBMP-2. The strategy of combining advanced scaffold fabrication technology, nanocomposite and growth factor delivery is promising for bone tissue regeneration.

Original languageEnglish (US)
Title of host publicationAdvances in Bioceramics and Porous Ceramics IV - A Collection of Papers Presented at the 35th International Conference on Advanced Ceramics and Composites, ICACC'11
Pages37-48
Number of pages12
Edition6
StatePublished - Dec 2 2011
EventAdvances in Bioceramics and Porous Ceramics IV - 35th International Conference on Advanced Ceramics and Composites, ICACC'11 - Daytona Beach, FL, United States
Duration: Jan 23 2011Jan 28 2011

Publication series

NameCeramic Engineering and Science Proceedings
Number6
Volume32
ISSN (Print)0196-6219

Other

OtherAdvances in Bioceramics and Porous Ceramics IV - 35th International Conference on Advanced Ceramics and Composites, ICACC'11
CountryUnited States
CityDaytona Beach, FL
Period1/23/111/28/11

Fingerprint

Scaffolds (biology)
Tissue engineering
Scaffolds
Nanocomposites
Bone
Proteins
Lasers
Stem cells
Heparin
Gelatin
Cell culture
Surface treatment
recombinant human bone morphogenetic protein-2
Tissue regeneration
Cell proliferation
Intercellular Signaling Peptides and Proteins
Sintering
Adsorption
Fabrication

ASJC Scopus subject areas

  • Ceramics and Composites
  • Materials Chemistry

Cite this

Duan, B., Lu, W. W., & Wang, M. (2011). Selective laser sintered Ca-P/PHBV nanocomposite scaffolds with sustained release of rhBMP-2 for bone tissue engineering. In Advances in Bioceramics and Porous Ceramics IV - A Collection of Papers Presented at the 35th International Conference on Advanced Ceramics and Composites, ICACC'11 (6 ed., pp. 37-48). (Ceramic Engineering and Science Proceedings; Vol. 32, No. 6).

Selective laser sintered Ca-P/PHBV nanocomposite scaffolds with sustained release of rhBMP-2 for bone tissue engineering. / Duan, Bin; Lu, William W.; Wang, Min.

Advances in Bioceramics and Porous Ceramics IV - A Collection of Papers Presented at the 35th International Conference on Advanced Ceramics and Composites, ICACC'11. 6. ed. 2011. p. 37-48 (Ceramic Engineering and Science Proceedings; Vol. 32, No. 6).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Duan, B, Lu, WW & Wang, M 2011, Selective laser sintered Ca-P/PHBV nanocomposite scaffolds with sustained release of rhBMP-2 for bone tissue engineering. in Advances in Bioceramics and Porous Ceramics IV - A Collection of Papers Presented at the 35th International Conference on Advanced Ceramics and Composites, ICACC'11. 6 edn, Ceramic Engineering and Science Proceedings, no. 6, vol. 32, pp. 37-48, Advances in Bioceramics and Porous Ceramics IV - 35th International Conference on Advanced Ceramics and Composites, ICACC'11, Daytona Beach, FL, United States, 1/23/11.
Duan B, Lu WW, Wang M. Selective laser sintered Ca-P/PHBV nanocomposite scaffolds with sustained release of rhBMP-2 for bone tissue engineering. In Advances in Bioceramics and Porous Ceramics IV - A Collection of Papers Presented at the 35th International Conference on Advanced Ceramics and Composites, ICACC'11. 6 ed. 2011. p. 37-48. (Ceramic Engineering and Science Proceedings; 6).
Duan, Bin ; Lu, William W. ; Wang, Min. / Selective laser sintered Ca-P/PHBV nanocomposite scaffolds with sustained release of rhBMP-2 for bone tissue engineering. Advances in Bioceramics and Porous Ceramics IV - A Collection of Papers Presented at the 35th International Conference on Advanced Ceramics and Composites, ICACC'11. 6. ed. 2011. pp. 37-48 (Ceramic Engineering and Science Proceedings; 6).
@inproceedings{d59b746258b74a7080d857257c171e64,
title = "Selective laser sintered Ca-P/PHBV nanocomposite scaffolds with sustained release of rhBMP-2 for bone tissue engineering",
abstract = "Ca-P/PHBV nanocomposite scaffolds for bone tissue engineering were fabricated via selective laser sintering. The surface modification of Ca-P/PHBV scaffolds was conducted firstly by physical entrapment of gelatin. Heparin was then immobilized on gelatin-modified scaffolds through covalent conjugation. Human umbilical cord derived mesenchymal stem cells (hUC-MSCs) were seeded onto the scaffolds. Compared to non-modified scaffolds, heparin-immobilized scaffolds exhibited higher cell proliferation at the early stage of cell culture. hUC-MSCs became confluent after 21 day culture on scaffolds and covered the whole scaffold surface, strongly adhering to the scaffolds. Recombinant human bone morphogenetic protein (rhBMP)-2 was loaded onto scaffolds with or without surface modification and its in vitro release behavior was studied. An initial burst release of rhBMP-2 was observed for both types of scaffolds. However, the immobilization of heparin on the surface of Ca-P/PHBV scaffolds not only provided a means to protect the rhBMP-2 but also improved its sustained release. Surface modified scaffolds loaded with rhBMP-2 promoted significantly higher ALP activity of hUC-MSCs than the scaffolds with simple adsorption of rhBMP-2. The strategy of combining advanced scaffold fabrication technology, nanocomposite and growth factor delivery is promising for bone tissue regeneration.",
author = "Bin Duan and Lu, {William W.} and Min Wang",
year = "2011",
month = "12",
day = "2",
language = "English (US)",
isbn = "9781118059913",
series = "Ceramic Engineering and Science Proceedings",
number = "6",
pages = "37--48",
booktitle = "Advances in Bioceramics and Porous Ceramics IV - A Collection of Papers Presented at the 35th International Conference on Advanced Ceramics and Composites, ICACC'11",
edition = "6",

}

TY - GEN

T1 - Selective laser sintered Ca-P/PHBV nanocomposite scaffolds with sustained release of rhBMP-2 for bone tissue engineering

AU - Duan, Bin

AU - Lu, William W.

AU - Wang, Min

PY - 2011/12/2

Y1 - 2011/12/2

N2 - Ca-P/PHBV nanocomposite scaffolds for bone tissue engineering were fabricated via selective laser sintering. The surface modification of Ca-P/PHBV scaffolds was conducted firstly by physical entrapment of gelatin. Heparin was then immobilized on gelatin-modified scaffolds through covalent conjugation. Human umbilical cord derived mesenchymal stem cells (hUC-MSCs) were seeded onto the scaffolds. Compared to non-modified scaffolds, heparin-immobilized scaffolds exhibited higher cell proliferation at the early stage of cell culture. hUC-MSCs became confluent after 21 day culture on scaffolds and covered the whole scaffold surface, strongly adhering to the scaffolds. Recombinant human bone morphogenetic protein (rhBMP)-2 was loaded onto scaffolds with or without surface modification and its in vitro release behavior was studied. An initial burst release of rhBMP-2 was observed for both types of scaffolds. However, the immobilization of heparin on the surface of Ca-P/PHBV scaffolds not only provided a means to protect the rhBMP-2 but also improved its sustained release. Surface modified scaffolds loaded with rhBMP-2 promoted significantly higher ALP activity of hUC-MSCs than the scaffolds with simple adsorption of rhBMP-2. The strategy of combining advanced scaffold fabrication technology, nanocomposite and growth factor delivery is promising for bone tissue regeneration.

AB - Ca-P/PHBV nanocomposite scaffolds for bone tissue engineering were fabricated via selective laser sintering. The surface modification of Ca-P/PHBV scaffolds was conducted firstly by physical entrapment of gelatin. Heparin was then immobilized on gelatin-modified scaffolds through covalent conjugation. Human umbilical cord derived mesenchymal stem cells (hUC-MSCs) were seeded onto the scaffolds. Compared to non-modified scaffolds, heparin-immobilized scaffolds exhibited higher cell proliferation at the early stage of cell culture. hUC-MSCs became confluent after 21 day culture on scaffolds and covered the whole scaffold surface, strongly adhering to the scaffolds. Recombinant human bone morphogenetic protein (rhBMP)-2 was loaded onto scaffolds with or without surface modification and its in vitro release behavior was studied. An initial burst release of rhBMP-2 was observed for both types of scaffolds. However, the immobilization of heparin on the surface of Ca-P/PHBV scaffolds not only provided a means to protect the rhBMP-2 but also improved its sustained release. Surface modified scaffolds loaded with rhBMP-2 promoted significantly higher ALP activity of hUC-MSCs than the scaffolds with simple adsorption of rhBMP-2. The strategy of combining advanced scaffold fabrication technology, nanocomposite and growth factor delivery is promising for bone tissue regeneration.

UR - http://www.scopus.com/inward/record.url?scp=82355175439&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=82355175439&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:82355175439

SN - 9781118059913

T3 - Ceramic Engineering and Science Proceedings

SP - 37

EP - 48

BT - Advances in Bioceramics and Porous Ceramics IV - A Collection of Papers Presented at the 35th International Conference on Advanced Ceramics and Composites, ICACC'11

ER -