Selective immunoprecipitate identification using monoclonal anti-rabbit IgG

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A monoclonal mouse antibody directed against rabbit IgG has been conjugated with horseradish peroxidase and used to identify immunoprecipitates which contain rabbit antibodies. By combining a specific rabbit antisera with a general antiserum from another species (e.g. goat antiserum against human serum), immunoprecipitates containing the antigen(s) recognized by the rabbit antibodies have been selectively identified by colorimetric development of peroxidase activity. Since the monoclonal antibody is specific for rabbit IgG and nonprecipitating, the peroxidase conjugatecan be included in the agarose with the primary antisera.

Original languageEnglish (US)
Pages (from-to)148-150
Number of pages3
JournalAnalytical Biochemistry
Volume145
Issue number1
DOIs
StatePublished - Feb 15 1985

Fingerprint

Immune Sera
Immunoglobulin G
Rabbits
Peroxidase
Antibodies
Monoclonal Antibodies
Horseradish Peroxidase
Sepharose
Goats
Antigens
anti-IgG
Serum

Keywords

  • horseradish peroxidase
  • immunoprecipitation
  • monoclonal antibody

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Selective immunoprecipitate identification using monoclonal anti-rabbit IgG. / Carson, Steven D.

In: Analytical Biochemistry, Vol. 145, No. 1, 15.02.1985, p. 148-150.

Research output: Contribution to journalArticle

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