Segregation of receptor and ligand regulates activation of epithelial growth factor receptor

Paola D. Vermeer, Lisa A. Einwalter, Thomas O. Moninger, Tatiana Rokhlina, Jeffrey A. Kern, Joseph Zabner, Michael J. Welsh

Research output: Contribution to journalArticle

268 Citations (Scopus)

Abstract

Interactions between ligands and receptors are central to communication between cells and tissues. Human airway epithelia constitutively produce both a ligand, the growth factor heregulin, and its receptors - erbB2, erbB3 and erbB4 (refs 1-3). Although heregulin binding initiates cellular proliferation and differentiation4-7, airway epithelia have a low rate of cell division8. This raises the question of how ligand-receptor interactions are controlled in epithelia. Here we show that in differentiated human airway epithelia, heregulin-α is present exclusively in the apical membrane and the overlying airway surface liquid, physically separated from erbB2-4, which segregate to the basolateral membrane. This physical arrangement creates a ligand-receptor pair poised for activation whenever epithelial integrity is disrupted. Indeed, immediately following a mechanical injury, heregulin-α activates erbB2 in cells at the edge of the wound, and this process hastens restoration of epithelial integrity. Likewise, when epithelial cells are not separated into apical and basolateral membranes ('polarized'), or when tight junctions between adjacent cells are opened, heregulin-α activates its receptor. This mechanism of ligand-receptor segregation on either side of epithelial tight junctions may be vital for rapid restoration of integrity following injury, and hence critical for survival. This model also suggests a mechanism for abnormal receptor activation in diseases with increased epithelial permeability.

Original languageEnglish (US)
Pages (from-to)322-326
Number of pages5
JournalNature
Volume422
Issue number6929
DOIs
StatePublished - Mar 20 2003

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Neuregulin-1
Growth Factor Receptors
Ligands
Epithelium
Tight Junctions
Membranes
Wounds and Injuries
Cell Communication
Permeability
Intercellular Signaling Peptides and Proteins
Epithelial Cells
Cell Proliferation
Survival

ASJC Scopus subject areas

  • General

Cite this

Vermeer, P. D., Einwalter, L. A., Moninger, T. O., Rokhlina, T., Kern, J. A., Zabner, J., & Welsh, M. J. (2003). Segregation of receptor and ligand regulates activation of epithelial growth factor receptor. Nature, 422(6929), 322-326. https://doi.org/10.1038/nature01440

Segregation of receptor and ligand regulates activation of epithelial growth factor receptor. / Vermeer, Paola D.; Einwalter, Lisa A.; Moninger, Thomas O.; Rokhlina, Tatiana; Kern, Jeffrey A.; Zabner, Joseph; Welsh, Michael J.

In: Nature, Vol. 422, No. 6929, 20.03.2003, p. 322-326.

Research output: Contribution to journalArticle

Vermeer, PD, Einwalter, LA, Moninger, TO, Rokhlina, T, Kern, JA, Zabner, J & Welsh, MJ 2003, 'Segregation of receptor and ligand regulates activation of epithelial growth factor receptor', Nature, vol. 422, no. 6929, pp. 322-326. https://doi.org/10.1038/nature01440
Vermeer PD, Einwalter LA, Moninger TO, Rokhlina T, Kern JA, Zabner J et al. Segregation of receptor and ligand regulates activation of epithelial growth factor receptor. Nature. 2003 Mar 20;422(6929):322-326. https://doi.org/10.1038/nature01440
Vermeer, Paola D. ; Einwalter, Lisa A. ; Moninger, Thomas O. ; Rokhlina, Tatiana ; Kern, Jeffrey A. ; Zabner, Joseph ; Welsh, Michael J. / Segregation of receptor and ligand regulates activation of epithelial growth factor receptor. In: Nature. 2003 ; Vol. 422, No. 6929. pp. 322-326.
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