Secondary structure of the ribonuclease H domain of the human immunodeficiency virus reverse transcriptase in solution using three-dimensional double and triple resonance heteronuclear magnetic resonance spectroscopy

Robert Powers, G. Marius Clore, Ad Bax, Daniel S. Garrett, Stephen J. Stahl, Paul T. Wingfield, Angela M. Gronenborn

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The solution structure of the ribonuclease H domain of HIV-1 reverse transcriptase has been investigated by three-dimensional double and triple resonance heteronuclear magnetic resonance spectroscopy. The domain studied has 138 residues and comprises residues 427 to 560 of the 66 kDa reverse transcriptase with an additional four residues at the N terminus. Initial studies on the wild-type protein were hindered by severe differential line broadening, presumably due to conformational averaging. Mutation of the single tryptophan residue located in a loop at position 113 (position 535 in the reverse transcriptase sequence) to an alanine resulted in much improved spectral properties with no apparent change in structure. 1H, 15N and 13C backbone resonances were assigned sequentially using a range of three-dimensional double and triple resonance heteronuclear experiments on samples of uniformly (>95%) 15N and 15N 13C-labeled protein, and the secondary structure was elucidated from a qualitative analysis of data derived from three-dimensional 15N- and 13C-edited nuclear Overhauser enhancement spectra. The secondary structure comprises three α-helices and five strands arranged in a mixed parallel/antiparallel β-sheet with a +1, +1, -3x, -1x topology. The C-terminal region from residue 114 onwards appears to be conformationally disordered in solution as evidenced by an almost complete absence of sequential and medium range nuclear Overhauser effects.

Original languageEnglish (US)
Pages (from-to)1081-1090
Number of pages10
JournalJournal of Molecular Biology
Volume221
Issue number4
DOIs
StatePublished - Oct 20 1991

Fingerprint

Ribonuclease H
HIV Reverse Transcriptase
RNA-Directed DNA Polymerase
Magnetic Resonance Spectroscopy
Secondary Protein Structure
Tryptophan
Alanine
Mutation
Proteins
Human immunodeficiency virus 1 reverse transcriptase

Keywords

  • 3D heteronuclear n.m.r.
  • HIV-1
  • RNase H domain
  • double resonance n.m.r.
  • reverse transcriptase
  • solution secondary structure
  • triple resonance n.m.r.

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

Cite this

Secondary structure of the ribonuclease H domain of the human immunodeficiency virus reverse transcriptase in solution using three-dimensional double and triple resonance heteronuclear magnetic resonance spectroscopy. / Powers, Robert; Clore, G. Marius; Bax, Ad; Garrett, Daniel S.; Stahl, Stephen J.; Wingfield, Paul T.; Gronenborn, Angela M.

In: Journal of Molecular Biology, Vol. 221, No. 4, 20.10.1991, p. 1081-1090.

Research output: Contribution to journalArticle

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