Searching for novel biomarkers using a mouse model of CLN3-Batten disease

Derek Timm, Jacob T. Cain, Ryan D. Geraets, Katherine A. White, Seung yon Koh, Tammy L Kielian, David A. Pearce, Michelle L. Hastings, Jill M. Weimer

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

CLN3-Batten disease is a rare, autosomal recessive disorder involving seizures, visual, motor and cognitive decline, and premature death. The Cln3 Δ ex7/8 mouse model recapitulates several phenotypic characteristics of the most common 1.02kb disease-associated deletion. Identification of reproducible biomarker(s) to facilitate longitudinal monitoring of disease progression and provide readouts for therapeutic response has remained elusive. One factor that has complicated the identification of suitable biomarkers in this mouse model has been that variations in animal husbandry appear to significantly influence readouts. In the current study, we cross-compared a number of biological parameters in blood from Cln3 Δ ex7/8 mice and control, non-disease mice on the same genetic background from multiple animal facilities in an attempt to better define a surrogate marker of CLN3-Batten disease. Interestingly, we found that significant differences between Batten and non-disease mice found at one site were generally not maintained across different facilities. Our results suggest that colony variation in the Cln3 Δ ex7/8 mouse model of CLN3-Batten disease can influence potential biomarkers of the disease.

Original languageEnglish (US)
Article numbere0201470
JournalPloS one
Volume13
Issue number8
DOIs
StatePublished - Aug 2018

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Neuronal Ceroid-Lipofuscinoses
Biomarkers
biomarkers
animal models
mice
animal husbandry
seizures
disease course
genetic background
Animals
Seizures
Animal Husbandry
death
therapeutics
Premature Mortality
monitoring
blood
Disease Progression
Epilepsy
Identification (control systems)

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Timm, D., Cain, J. T., Geraets, R. D., White, K. A., Koh, S. Y., Kielian, T. L., ... Weimer, J. M. (2018). Searching for novel biomarkers using a mouse model of CLN3-Batten disease. PloS one, 13(8), [e0201470]. https://doi.org/10.1371/journal.pone.0201470

Searching for novel biomarkers using a mouse model of CLN3-Batten disease. / Timm, Derek; Cain, Jacob T.; Geraets, Ryan D.; White, Katherine A.; Koh, Seung yon; Kielian, Tammy L; Pearce, David A.; Hastings, Michelle L.; Weimer, Jill M.

In: PloS one, Vol. 13, No. 8, e0201470, 08.2018.

Research output: Contribution to journalArticle

Timm, D, Cain, JT, Geraets, RD, White, KA, Koh, SY, Kielian, TL, Pearce, DA, Hastings, ML & Weimer, JM 2018, 'Searching for novel biomarkers using a mouse model of CLN3-Batten disease', PloS one, vol. 13, no. 8, e0201470. https://doi.org/10.1371/journal.pone.0201470
Timm, Derek ; Cain, Jacob T. ; Geraets, Ryan D. ; White, Katherine A. ; Koh, Seung yon ; Kielian, Tammy L ; Pearce, David A. ; Hastings, Michelle L. ; Weimer, Jill M. / Searching for novel biomarkers using a mouse model of CLN3-Batten disease. In: PloS one. 2018 ; Vol. 13, No. 8.
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