Screening of anti-MUC1 antibodies for reactivity with native (ascites) and recombinant (baculovirus) MUC1 and for blocking MUC1 specific cytotoxic T-lymphocytes

Pawel S Ciborowski, William M. Konitzki, Julie Magarian Blander, Olivera J. Finn

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We report the results of a panel of 5 monoclonal antibodies submitted to the ISOBM TD-4 Workshop for the study of the epithelial MUC1 mucin. We used three forms of the MUC1 as antigen. One form of mucin was the native, highly glycosylated MUC1 isolated from the ascites of breast or pancreatic cancer patients. Two other forms of the mucin were recombinantly expressed in a baculovirus expression system as either fully glycosylated, or underglycosylated. Based on the results of Western blot analysis we were able to group the antibodies into 7 clusters depending on their recognition of the MUC1 forms tested. We then selected several antibodies, representatives of each cluster, to test for the ability to block MUC1-specific cytotoxic T- lymphocyte (CTL) function. We found that antibodies ISOBM-163 and ISOBM-147 blocked cytotoxicity of MUC1-specific CTL against two tumor targets in a concentration-dependent manner.

Original languageEnglish (US)
Pages (from-to)147-151
Number of pages5
JournalTumor Biology
Volume19
Issue numberSUPPL.1
DOIs
StatePublished - Dec 1 1997

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Baculoviridae
Cytotoxic T-Lymphocytes
Mucins
Ascites
Anti-Idiotypic Antibodies
Antibodies
Pancreatic Neoplasms
Western Blotting
Monoclonal Antibodies
Breast Neoplasms
Education
Antigens
Neoplasms

Keywords

  • MUC1
  • Monoclonal antibody
  • TD-4 Workshop

ASJC Scopus subject areas

  • Cancer Research

Cite this

Screening of anti-MUC1 antibodies for reactivity with native (ascites) and recombinant (baculovirus) MUC1 and for blocking MUC1 specific cytotoxic T-lymphocytes. / Ciborowski, Pawel S; Konitzki, William M.; Blander, Julie Magarian; Finn, Olivera J.

In: Tumor Biology, Vol. 19, No. SUPPL.1, 01.12.1997, p. 147-151.

Research output: Contribution to journalArticle

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AB - We report the results of a panel of 5 monoclonal antibodies submitted to the ISOBM TD-4 Workshop for the study of the epithelial MUC1 mucin. We used three forms of the MUC1 as antigen. One form of mucin was the native, highly glycosylated MUC1 isolated from the ascites of breast or pancreatic cancer patients. Two other forms of the mucin were recombinantly expressed in a baculovirus expression system as either fully glycosylated, or underglycosylated. Based on the results of Western blot analysis we were able to group the antibodies into 7 clusters depending on their recognition of the MUC1 forms tested. We then selected several antibodies, representatives of each cluster, to test for the ability to block MUC1-specific cytotoxic T- lymphocyte (CTL) function. We found that antibodies ISOBM-163 and ISOBM-147 blocked cytotoxicity of MUC1-specific CTL against two tumor targets in a concentration-dependent manner.

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