Scid mice with hiv encephalitis develop behavioral abnormalities

W. Tyor, B. Keliey, N. Avgeropoulos, L. Middaugh, Y. Persidsky, Howard Eliot Gendelman, S. Arrigo

Research output: Contribution to journalArticle

Abstract

SCID mice inoculated intracerebrally (1C) with preinfected (HIV) human macrophages develop brain pathology similar to that in humans with HIV encephalitis. This includes immimocytochemical detection in brain sections of HIV-infected macrophages and multinucleated giant cells, and significant astrogliosis compared to controls (mice injected 1C with uninfected human macrophages). These xenografts survive about 1 month. To develop a model of chronic HIV encephalitis and determine if chronically infected mice develop behavioral abnormalities similar to the human condition, we reinoculated SCID mice 1C every 4 weeks with either preinfected human macrophages (infected mice), uninfected human macrophages, or no inoculation; these groups were monitored for behavioral abnormalities. For 3 months after the first inoculation (total of 4 inoculations) each mouse was monitored on Stoelting activity monitors for 24 hours twice a month. There was no significant difference between groups in the amount of general activity during their normal day to day routine. However, the acquisition of a Morris maze problem by infected mice 3 1/2 months after the first inoculation was deficient in comparison to mice inoculated with uninfected macrophages or uninoculated mice. Ongoing studies are investigating the potential relationships between:(l) the histopathology such as the presence and amount of macrophages, HIV, and astrogliosis, (2) cytokine production (in situ detection and quantitative PCR for mllNA) such as TNF-alpha and others, and (3) the behavioral abnormalities.

Original languageEnglish (US)
Pages (from-to)A1336
JournalFASEB Journal
Volume10
Issue number6
StatePublished - Dec 1 1996

Fingerprint

Macrophages
Encephalitis
encephalitis
macrophages
mice
HIV
SCID Mice
Brain
HIV-2
Pathology
Giant Cells
brain
Heterografts
giant cells
Tumor Necrosis Factor-alpha
histopathology
tumor necrosis factor-alpha
Cytokines
quantitative polymerase chain reaction
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Tyor, W., Keliey, B., Avgeropoulos, N., Middaugh, L., Persidsky, Y., Gendelman, H. E., & Arrigo, S. (1996). Scid mice with hiv encephalitis develop behavioral abnormalities. FASEB Journal, 10(6), A1336.

Scid mice with hiv encephalitis develop behavioral abnormalities. / Tyor, W.; Keliey, B.; Avgeropoulos, N.; Middaugh, L.; Persidsky, Y.; Gendelman, Howard Eliot; Arrigo, S.

In: FASEB Journal, Vol. 10, No. 6, 01.12.1996, p. A1336.

Research output: Contribution to journalArticle

Tyor, W, Keliey, B, Avgeropoulos, N, Middaugh, L, Persidsky, Y, Gendelman, HE & Arrigo, S 1996, 'Scid mice with hiv encephalitis develop behavioral abnormalities', FASEB Journal, vol. 10, no. 6, pp. A1336.
Tyor W, Keliey B, Avgeropoulos N, Middaugh L, Persidsky Y, Gendelman HE et al. Scid mice with hiv encephalitis develop behavioral abnormalities. FASEB Journal. 1996 Dec 1;10(6):A1336.
Tyor, W. ; Keliey, B. ; Avgeropoulos, N. ; Middaugh, L. ; Persidsky, Y. ; Gendelman, Howard Eliot ; Arrigo, S. / Scid mice with hiv encephalitis develop behavioral abnormalities. In: FASEB Journal. 1996 ; Vol. 10, No. 6. pp. A1336.
@article{c1e08fb1566746fcaded717c19bc1598,
title = "Scid mice with hiv encephalitis develop behavioral abnormalities",
abstract = "SCID mice inoculated intracerebrally (1C) with preinfected (HIV) human macrophages develop brain pathology similar to that in humans with HIV encephalitis. This includes immimocytochemical detection in brain sections of HIV-infected macrophages and multinucleated giant cells, and significant astrogliosis compared to controls (mice injected 1C with uninfected human macrophages). These xenografts survive about 1 month. To develop a model of chronic HIV encephalitis and determine if chronically infected mice develop behavioral abnormalities similar to the human condition, we reinoculated SCID mice 1C every 4 weeks with either preinfected human macrophages (infected mice), uninfected human macrophages, or no inoculation; these groups were monitored for behavioral abnormalities. For 3 months after the first inoculation (total of 4 inoculations) each mouse was monitored on Stoelting activity monitors for 24 hours twice a month. There was no significant difference between groups in the amount of general activity during their normal day to day routine. However, the acquisition of a Morris maze problem by infected mice 3 1/2 months after the first inoculation was deficient in comparison to mice inoculated with uninfected macrophages or uninoculated mice. Ongoing studies are investigating the potential relationships between:(l) the histopathology such as the presence and amount of macrophages, HIV, and astrogliosis, (2) cytokine production (in situ detection and quantitative PCR for mllNA) such as TNF-alpha and others, and (3) the behavioral abnormalities.",
author = "W. Tyor and B. Keliey and N. Avgeropoulos and L. Middaugh and Y. Persidsky and Gendelman, {Howard Eliot} and S. Arrigo",
year = "1996",
month = "12",
day = "1",
language = "English (US)",
volume = "10",
pages = "A1336",
journal = "FASEB Journal",
issn = "0892-6638",
publisher = "FASEB",
number = "6",

}

TY - JOUR

T1 - Scid mice with hiv encephalitis develop behavioral abnormalities

AU - Tyor, W.

AU - Keliey, B.

AU - Avgeropoulos, N.

AU - Middaugh, L.

AU - Persidsky, Y.

AU - Gendelman, Howard Eliot

AU - Arrigo, S.

PY - 1996/12/1

Y1 - 1996/12/1

N2 - SCID mice inoculated intracerebrally (1C) with preinfected (HIV) human macrophages develop brain pathology similar to that in humans with HIV encephalitis. This includes immimocytochemical detection in brain sections of HIV-infected macrophages and multinucleated giant cells, and significant astrogliosis compared to controls (mice injected 1C with uninfected human macrophages). These xenografts survive about 1 month. To develop a model of chronic HIV encephalitis and determine if chronically infected mice develop behavioral abnormalities similar to the human condition, we reinoculated SCID mice 1C every 4 weeks with either preinfected human macrophages (infected mice), uninfected human macrophages, or no inoculation; these groups were monitored for behavioral abnormalities. For 3 months after the first inoculation (total of 4 inoculations) each mouse was monitored on Stoelting activity monitors for 24 hours twice a month. There was no significant difference between groups in the amount of general activity during their normal day to day routine. However, the acquisition of a Morris maze problem by infected mice 3 1/2 months after the first inoculation was deficient in comparison to mice inoculated with uninfected macrophages or uninoculated mice. Ongoing studies are investigating the potential relationships between:(l) the histopathology such as the presence and amount of macrophages, HIV, and astrogliosis, (2) cytokine production (in situ detection and quantitative PCR for mllNA) such as TNF-alpha and others, and (3) the behavioral abnormalities.

AB - SCID mice inoculated intracerebrally (1C) with preinfected (HIV) human macrophages develop brain pathology similar to that in humans with HIV encephalitis. This includes immimocytochemical detection in brain sections of HIV-infected macrophages and multinucleated giant cells, and significant astrogliosis compared to controls (mice injected 1C with uninfected human macrophages). These xenografts survive about 1 month. To develop a model of chronic HIV encephalitis and determine if chronically infected mice develop behavioral abnormalities similar to the human condition, we reinoculated SCID mice 1C every 4 weeks with either preinfected human macrophages (infected mice), uninfected human macrophages, or no inoculation; these groups were monitored for behavioral abnormalities. For 3 months after the first inoculation (total of 4 inoculations) each mouse was monitored on Stoelting activity monitors for 24 hours twice a month. There was no significant difference between groups in the amount of general activity during their normal day to day routine. However, the acquisition of a Morris maze problem by infected mice 3 1/2 months after the first inoculation was deficient in comparison to mice inoculated with uninfected macrophages or uninoculated mice. Ongoing studies are investigating the potential relationships between:(l) the histopathology such as the presence and amount of macrophages, HIV, and astrogliosis, (2) cytokine production (in situ detection and quantitative PCR for mllNA) such as TNF-alpha and others, and (3) the behavioral abnormalities.

UR - http://www.scopus.com/inward/record.url?scp=33749115445&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33749115445&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:33749115445

VL - 10

SP - A1336

JO - FASEB Journal

JF - FASEB Journal

SN - 0892-6638

IS - 6

ER -