Scalable Culturing of Primary Human Glioblastoma Tumor-Initiating Cells with a Cell-Friendly Culture System

Qiang Li, Haishuang Lin, Jack Rauch, Loic P. Deleyrolle, Brent A. Reynolds, Hendrik J Viljoen, Chi Zhang, Linxia Gu, Erika Van Wyk, Yuguo Lei

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Glioblastoma is the most aggressive and deadly brain cancer. There is growing interest to develop drugs that specifically target to glioblastoma tumor-initiating cells (TICs). However, the cost-effective production of large numbers of high quality glioblastoma TICs for drug discovery with current cell culturing technologies remains very challenging. Here, we report a new method that cultures glioblastoma TICs in microscale alginate hydrogel tubes (or AlgTubes). The AlgTubes allowed long-term culturing (∼50 days, 10 passages) of glioblastoma TICs with high growth rate (∼700-fold expansion/14 days), high cell viability and high volumetric yield (∼3.0 × 108 cells/mL) without losing the stem cell properties, all offered large advancements over current culturing methods. This method can be applied for the scalable production of glioblastoma TICs at affordable cost for drug discovery.

Original languageEnglish (US)
Article number3531
JournalScientific reports
Volume8
Issue number1
DOIs
StatePublished - Dec 1 2018

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Neoplastic Stem Cells
Glioblastoma
Cell Culture Techniques
Drug Discovery
Costs and Cost Analysis
Hydrogel
Brain Neoplasms
Cell Survival
Stem Cells
Technology
Growth
Pharmaceutical Preparations

ASJC Scopus subject areas

  • General

Cite this

Scalable Culturing of Primary Human Glioblastoma Tumor-Initiating Cells with a Cell-Friendly Culture System. / Li, Qiang; Lin, Haishuang; Rauch, Jack; Deleyrolle, Loic P.; Reynolds, Brent A.; Viljoen, Hendrik J; Zhang, Chi; Gu, Linxia; Van Wyk, Erika; Lei, Yuguo.

In: Scientific reports, Vol. 8, No. 1, 3531, 01.12.2018.

Research output: Contribution to journalArticle

Li, Qiang ; Lin, Haishuang ; Rauch, Jack ; Deleyrolle, Loic P. ; Reynolds, Brent A. ; Viljoen, Hendrik J ; Zhang, Chi ; Gu, Linxia ; Van Wyk, Erika ; Lei, Yuguo. / Scalable Culturing of Primary Human Glioblastoma Tumor-Initiating Cells with a Cell-Friendly Culture System. In: Scientific reports. 2018 ; Vol. 8, No. 1.
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