Salt‐dependent conformational transitions in the self‐complementary deoxydodecanucleotide d(CGCAATTCGCG): Evidence for hairpin formation

Luis A. Marky, Kenneth S. Blumenfeld, Sharon Kozlowski, Kenneth J. Breslauer

Research output: Contribution to journalArticle

235 Citations (Scopus)

Abstract

Differential scanning calorimetry (DSC), temperature‐dependent uv‐absorption spectroscopy, and temperature‐dependent CD were used to monitor and characterize the salt‐dependent, thermally induced structural transitions in the deoxydodecanucleotide d(CGCGAATTCGCG). At the high oligomer concentrations required for DSC, the calorimetric scans revealed a single, monophasic transition curve at all salt concentrations. Based on previous nmr melting studies under similar conditions, we conclude that these monophasic transitions correspond to the cooperative duplex‐to‐single‐strand conversion of the dodecamer. By contrast, at the lower oligomer concentrations used for the spectroscopic studies, the shapes of the uv and CD melting curves were found to depend on the concentration of the added salt. At high salt (≥0.1M Na+), a single, monophasic transition curve was observed. At lower salt (⩽0.01M Na+), the CD and uv melting curves exhibit biphasic behavior. Based on the concentration dependence, the enthalpy, and the cooperativity of each transition in the biphasic curve, we conclude that at low salt and low oligomer concentrations, the dodecamer melts in a sequential manner involving initial disruption of a duplex structure and subsequent disruption of a hairpin structure.

Original languageEnglish (US)
Pages (from-to)1247-1257
Number of pages11
JournalBiopolymers
Volume22
Issue number4
DOIs
StatePublished - Apr 1983

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Salts
Oligomers
Freezing
Melting
Differential Scanning Calorimetry
Differential scanning calorimetry
Enthalpy
Spectrum Analysis
Spectroscopy

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Biomaterials
  • Organic Chemistry

Cite this

Salt‐dependent conformational transitions in the self‐complementary deoxydodecanucleotide d(CGCAATTCGCG) : Evidence for hairpin formation. / Marky, Luis A.; Blumenfeld, Kenneth S.; Kozlowski, Sharon; Breslauer, Kenneth J.

In: Biopolymers, Vol. 22, No. 4, 04.1983, p. 1247-1257.

Research output: Contribution to journalArticle

Marky, Luis A. ; Blumenfeld, Kenneth S. ; Kozlowski, Sharon ; Breslauer, Kenneth J. / Salt‐dependent conformational transitions in the self‐complementary deoxydodecanucleotide d(CGCAATTCGCG) : Evidence for hairpin formation. In: Biopolymers. 1983 ; Vol. 22, No. 4. pp. 1247-1257.
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