Salmonella enterica in swine production: Assessing the association between amplified fragment length polymorphism and epidemiological units of concern

Bing Wang, Chong Wang, James D. McKean, Catherine M. Logue, Wondwossen A. Gebreyes, Kelly A. Tivendale, Annette M. O'Connor

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The aims of this study were to determine the ability of amplified fragment length polymorphism (AFLP) to differentiate Salmonella isolates from different units of swine production and to demonstrate the relatedness of Salmonella between farms and abattoirs by AFLP. Twenty-four farms in the midwestern United States were visited four times from 2006 to 2009. At each farm or abattoir visit, 30 fecal samples or 30 mesenteric lymph nodes were collected, respectively. A total of 220 Salmonella isolates were obtained, serotyped, and genotyped by multilocus sequence typing (MLST) and AFLP. These 220 isolates clustered into 21 serotypes, 18 MLST types, and 14 predominant AFLP clusters based on a genetic similarity threshold level of 60%. To assess genetic differentiation between farms, harvest cohorts, and pigs, analysis of molecular variance was conducted using AFLP data. The results showed 65.62% of overall genetic variation was attributed to variance among pigs, 27.21% to farms, and 7.17% to harvest cohorts. Variance components at the farm (P = 0.003) and pig (P = 0.001) levels were significant, but not at the harvest cohort level (P = 0.079). A second analysis, a permutation test using AFLP data, indicated that on-farm and at-abattoir Salmonella from pigs of the same farms were more related than from different farms. Therefore, among the three subtyping methods, serotyping, MLST, and AFLP, AFLP was the method that was able to differentiate among Salmonella isolates from different farms and link contamination at the abattoir to the farm of origin.

Original languageEnglish (US)
Pages (from-to)8080-8087
Number of pages8
JournalApplied and environmental microbiology
Volume77
Issue number22
DOIs
StatePublished - Nov 1 2011

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Salmonella enterica
amplified fragment length polymorphism
polymorphism
Swine
farm
farms
Abattoirs
Salmonella
Multilocus Sequence Typing
pig
slaughterhouses
swine
swine production
Farms
salmonella
Midwestern United States
Serotyping
genetic variation
relatedness
genetic differentiation

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

Cite this

Salmonella enterica in swine production : Assessing the association between amplified fragment length polymorphism and epidemiological units of concern. / Wang, Bing; Wang, Chong; McKean, James D.; Logue, Catherine M.; Gebreyes, Wondwossen A.; Tivendale, Kelly A.; O'Connor, Annette M.

In: Applied and environmental microbiology, Vol. 77, No. 22, 01.11.2011, p. 8080-8087.

Research output: Contribution to journalArticle

Wang, Bing ; Wang, Chong ; McKean, James D. ; Logue, Catherine M. ; Gebreyes, Wondwossen A. ; Tivendale, Kelly A. ; O'Connor, Annette M. / Salmonella enterica in swine production : Assessing the association between amplified fragment length polymorphism and epidemiological units of concern. In: Applied and environmental microbiology. 2011 ; Vol. 77, No. 22. pp. 8080-8087.
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