Safety assessment of the calcium-binding protein, apoaequorin, expressed by Escherichia coli

Daniel L. Moran, Afua O. Tetteh, Richard E. Goodman, Mark Y. Underwood

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Calcium-binding proteins are ubiquitous modulators of cellular activity and function. Cells possess numerous calcium-binding proteins that regulate calcium concentration in the cytosol by buffering excess free calcium ion. Disturbances in intracellular calcium homeostasis are at the heart of many age-related conditions making these proteins targets for therapeutic intervention. A calcium-binding protein, apoaequorin, has shown potential utility in a broad spectrum of applications for human health and well-being. Large-scale recombinant production of the protein has been successful; enabling further research and development and commercialization efforts. Previous work reported a 90-day subchronic toxicity test that demonstrated this protein has no toxicity by oral exposure in Sprague-Dawley rodents. The current study assesses the allergenic potential of the purified protein using bioinformatic analysis and simulated gastric digestion. The results from the bioinformatics searches with the apoaequorin sequence show the protein is not a known allergen and not likely to cross-react with known allergens. Apoaequorin is easily digested by pepsin, a characteristic commonly exhibited by many non-allergenic dietary proteins. From these data, there is no added concern of safety due to unusual stability of the protein by ingestion.

Original languageEnglish (US)
Pages (from-to)243-249
Number of pages7
JournalRegulatory Toxicology and Pharmacology
Volume69
Issue number2
DOIs
StatePublished - Jul 2014

Fingerprint

Calcium-Binding Proteins
Escherichia coli
Safety
Calcium
Computational Biology
Allergens
Subchronic Toxicity Tests
Proteins
Bioinformatics
Technology Transfer
Toxicity
Dietary Proteins
Protein Stability
Pepsin A
Recombinant Proteins
Cytosol
Digestion
Rodentia
Stomach
Homeostasis

Keywords

  • Allergenicity
  • Apoaequorin
  • Bioinformatic
  • Calcium-binding protein
  • Safety assessment

ASJC Scopus subject areas

  • Toxicology

Cite this

Safety assessment of the calcium-binding protein, apoaequorin, expressed by Escherichia coli. / Moran, Daniel L.; Tetteh, Afua O.; Goodman, Richard E.; Underwood, Mark Y.

In: Regulatory Toxicology and Pharmacology, Vol. 69, No. 2, 07.2014, p. 243-249.

Research output: Contribution to journalArticle

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