Ryanodine receptor expression is associated with intracellular Ca2+ release in rat parotid acinar cells

Xuejun Zhang, Jiayu Wen, Keshore R. Bidasee, Henry R. Besch, Ronald P. Rubin

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The ryanodine receptor mediates intracellular Ca2+ mobilization in muscle and nerve, but its physiological role in nonexcitable cells is less well defined. Like adenosine 3',5'-cyclic monophosphate and inositol 1,4,5- trisphosphate, cyclic ADP-ribose (0.3-5 μM) and ADP (1-25 μM) produced a concentration-dependent rise in cytosolic Ca2+ in permeabilized rat parotid acinar cells. Adenosine and AMP were less effective. Ryanodine markedly depressed the Ca2+-mobilizing action of the adenine nucleotides and forskolin in permeabilized cells and was likewise effective in depressing the action of forskolin in intact cells. Cyclic ADP-ribose-evoked Ca2+ release was enhanced by calmodulin and depressed by W-7, a calmodulin inhibitor. A fluorescently labeled ligand, 4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3,4- diaza-s-indacene-3-propionic acid-glycyl ryanodine, was synthesized to detect the expression and distribution of ryanodine receptors. In addition, ryanodine receptor expression was detected in rat parotid cells with a sequence highly homologous to a rat skeletal muscle type i and a novel brain type 1 ryanodine receptor. These findings demonstrate the presence of a ryanodine-sensitive intracellular Ca2+ store in rat parotid cells that shares many of the characteristics of stores in muscle and nerve and may mediate Ca2+-induced Ca2+ release or a modified form of this process.

Original languageEnglish (US)
Pages (from-to)C1306-C1314
JournalAmerican Journal of Physiology - Cell Physiology
Volume273
Issue number4 42-4
StatePublished - Nov 19 1997

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Ryanodine Receptor Calcium Release Channel
Acinar Cells
Ryanodine
Rats
Cyclic ADP-Ribose
Muscle
Colforsin
Calmodulin
Inositol 1,4,5-Trisphosphate
Adenine Nucleotides
Adenosine Monophosphate
Muscles
Cyclic AMP
Adenosine
Adenosine Diphosphate
Brain
Sequence Homology
Ligands
Skeletal Muscle

Keywords

  • Adenosine 3',5'-cyclic monophosphate
  • Cyclic adenosine diphosphate-ribose

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

Cite this

Ryanodine receptor expression is associated with intracellular Ca2+ release in rat parotid acinar cells. / Zhang, Xuejun; Wen, Jiayu; Bidasee, Keshore R.; Besch, Henry R.; Rubin, Ronald P.

In: American Journal of Physiology - Cell Physiology, Vol. 273, No. 4 42-4, 19.11.1997, p. C1306-C1314.

Research output: Contribution to journalArticle

Zhang, Xuejun ; Wen, Jiayu ; Bidasee, Keshore R. ; Besch, Henry R. ; Rubin, Ronald P. / Ryanodine receptor expression is associated with intracellular Ca2+ release in rat parotid acinar cells. In: American Journal of Physiology - Cell Physiology. 1997 ; Vol. 273, No. 4 42-4. pp. C1306-C1314.
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