Rubisco activase and wheat productivity under heat-stress conditions

Zoran Ristic, Ivana Momilović, Urka Bukovnik, P. V.Vara Prasad, Jianming Fu, Benjamin P. Deridder, Thomas E. Elthon, Novica Mladenov

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Rubisco activase (RCA) constrains the photosynthetic potential of plants at high temperatures (heat stress). Endogenous levels of RCA could serve as an important determinant of plant productivity under heat-stress conditions. Thus, in this study, the possible relationship between expression levels of RCA and plant yield in 11 European cultivars of winter wheat following prolonged exposure to heat stress was investigated. In addition, the effect of a short-term heat stress on RCA expression in four genotypes of wheat, five genotypes of maize, and one genotype of Arabidopsis thaliana was examined. Immunoblots prepared from leaf protein extracts from control plants showed three RCA cross-reacting bands in wheat and two RCA cross-reacting bands in maize and Arabidopsis. The molecular mass of the observed bands was in the range between 40 kDa and 46 kDa. Heat stress affected RCA expression in a few genotypes of wheat and maize but not in Arabidopsis. In wheat, heat stress slightly modulated the relative amounts of RCA in some cultivars. In maize, heat stress did not seem to affect the existing RCA isoforms (40 kDa and 43 kDa) but induced the accumulation of a new putative RCA of 45-46 kDa. The new putative 45-46 kDa RCA was not seen in a genotype of maize (ZPL 389) that has been shown to display an exceptional sensitivity to heat stress. A significant, positive, linear correlation was found between the expression of wheat 45-46 kDa RCA and plant productivity under heat-stress conditions. Results support the hypothesis that endogenous levels of RCA could play an important role in plant productivity under supraoptimal temperature conditions.

Original languageEnglish (US)
Pages (from-to)4003-4014
Number of pages12
JournalJournal of experimental botany
Volume60
Issue number14
DOIs
StatePublished - Oct 1 2009

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Ribulose-Bisphosphate Carboxylase
Tissue Plasminogen Activator
Triticum
heat stress
Hot Temperature
wheat
Zea mays
Genotype
Arabidopsis
corn
genotype
ribulose-bisphosphate carboxylase activase
Temperature
Plant Extracts
leaf protein
cultivars
Protein Isoforms

Keywords

  • Heat stress
  • Heat tolerance
  • Rubisco activase
  • Wheat productivity

ASJC Scopus subject areas

  • Physiology
  • Plant Science

Cite this

Ristic, Z., Momilović, I., Bukovnik, U., Prasad, P. V. V., Fu, J., Deridder, B. P., ... Mladenov, N. (2009). Rubisco activase and wheat productivity under heat-stress conditions. Journal of experimental botany, 60(14), 4003-4014. https://doi.org/10.1093/jxb/erp241

Rubisco activase and wheat productivity under heat-stress conditions. / Ristic, Zoran; Momilović, Ivana; Bukovnik, Urka; Prasad, P. V.Vara; Fu, Jianming; Deridder, Benjamin P.; Elthon, Thomas E.; Mladenov, Novica.

In: Journal of experimental botany, Vol. 60, No. 14, 01.10.2009, p. 4003-4014.

Research output: Contribution to journalArticle

Ristic, Z, Momilović, I, Bukovnik, U, Prasad, PVV, Fu, J, Deridder, BP, Elthon, TE & Mladenov, N 2009, 'Rubisco activase and wheat productivity under heat-stress conditions', Journal of experimental botany, vol. 60, no. 14, pp. 4003-4014. https://doi.org/10.1093/jxb/erp241
Ristic Z, Momilović I, Bukovnik U, Prasad PVV, Fu J, Deridder BP et al. Rubisco activase and wheat productivity under heat-stress conditions. Journal of experimental botany. 2009 Oct 1;60(14):4003-4014. https://doi.org/10.1093/jxb/erp241
Ristic, Zoran ; Momilović, Ivana ; Bukovnik, Urka ; Prasad, P. V.Vara ; Fu, Jianming ; Deridder, Benjamin P. ; Elthon, Thomas E. ; Mladenov, Novica. / Rubisco activase and wheat productivity under heat-stress conditions. In: Journal of experimental botany. 2009 ; Vol. 60, No. 14. pp. 4003-4014.
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