RpiR homologues may link Staphylococcus aureus RNAIII synthesis and pentose phosphate pathway regulation

Yefei Zhu, Renu Nandakumar, Marat R. Sadykov, Nandakumar Madayiputhiya, Thanh T. Luong, Rosmarie Gaupp, Chia Y. Lee, Greg A Somerville

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Staphylococcus aureus is a medically important pathogen that synthesizes a wide range of virulence determinants. The synthesis of many staphylococcal virulence determinants is regulated in part by stress-induced changes in the activity of the tricarboxylic acid (TCA) cycle. One metabolic change associated with TCA cycle stress is an increased concentration of ribose, leading us to hypothesize that a pentose phosphate pathway (PPP)-responsive regulator mediates some of the TCA cycle-dependent regulatory effects. Using bioinformatics, we identified three potential ribose-responsive regulators that belong to the RpiR family of transcriptional regulators. To determine whether these RpiR homologues affect PPP activity and virulence determinant synthesis, the rpiR homologues were inactivated, and the effects on PPP activity and virulence factor synthesis were assessed. Two of the three homologues (RpiRB and RpiRC) positively influence the transcription of the PPP genes rpiA and zwf, while the third homologue (RpiRA) is slightly antagonistic to the other homologues. In addition, inactivation of RpiRC altered the temporal transcription of RNAIII, the effector molecule of the agr quorum-sensing system. These data confirm the close linkage of central metabolism and virulence determinant synthesis, and they establish a metabolic override for quorum-sensing-dependent regulation of RNAIII transcription.

Original languageEnglish (US)
Pages (from-to)6187-6196
Number of pages10
JournalJournal of bacteriology
Volume193
Issue number22
DOIs
StatePublished - Nov 1 2011

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Pentose Phosphate Pathway
Citric Acid Cycle
Virulence
Quorum Sensing
Ribose
Virulence Factors
Computational Biology
Staphylococcus aureus
Staphylococcus aureus RNAIII
Genes

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

RpiR homologues may link Staphylococcus aureus RNAIII synthesis and pentose phosphate pathway regulation. / Zhu, Yefei; Nandakumar, Renu; Sadykov, Marat R.; Madayiputhiya, Nandakumar; Luong, Thanh T.; Gaupp, Rosmarie; Lee, Chia Y.; Somerville, Greg A.

In: Journal of bacteriology, Vol. 193, No. 22, 01.11.2011, p. 6187-6196.

Research output: Contribution to journalArticle

Zhu, Y, Nandakumar, R, Sadykov, MR, Madayiputhiya, N, Luong, TT, Gaupp, R, Lee, CY & Somerville, GA 2011, 'RpiR homologues may link Staphylococcus aureus RNAIII synthesis and pentose phosphate pathway regulation', Journal of bacteriology, vol. 193, no. 22, pp. 6187-6196. https://doi.org/10.1128/JB.05930-11
Zhu Y, Nandakumar R, Sadykov MR, Madayiputhiya N, Luong TT, Gaupp R et al. RpiR homologues may link Staphylococcus aureus RNAIII synthesis and pentose phosphate pathway regulation. Journal of bacteriology. 2011 Nov 1;193(22):6187-6196. https://doi.org/10.1128/JB.05930-11
Zhu, Yefei ; Nandakumar, Renu ; Sadykov, Marat R. ; Madayiputhiya, Nandakumar ; Luong, Thanh T. ; Gaupp, Rosmarie ; Lee, Chia Y. ; Somerville, Greg A. / RpiR homologues may link Staphylococcus aureus RNAIII synthesis and pentose phosphate pathway regulation. In: Journal of bacteriology. 2011 ; Vol. 193, No. 22. pp. 6187-6196.
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