ROS-induced nanotherapeutic approach for ovarian cancer treatment based on the combinatorial effect of photodynamic therapy and DJ-1 gene suppression

Canan Schumann, Olena Taratula, Oleh Khalimonchuk, Amy L. Palmer, Lauren M. Cronk, Carson V. Jones, Cesar A. Escalante, Oleh Taratula

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

This study represents a novel approach for intraoperative ovarian cancer treatment based on the combinatorial effect of a targeted photodynamic therapy (PDT) associated with suppression of the DJ-1 protein, one of the key players in the ROS defense of cancer cells. To assess the potential of the developed therapy, dendrimer-based nanoplatforms for cancer-targeted delivery of near-infrared photosensitizer, phthalocyanine, and DJ-1 siRNA have been constructed. In vitro studies revealed that therapeutic efficacy of the combinatorial approach was enhanced when compared to PDT alone and this enhancement was more pronounced in ovarian carcinoma cells, which are characterized by higher basal levels of DJ-1 protein. Moreover, the ovarian cancer tumors exposed to a single dose of combinatorial therapy were completely eradicated from the mice and the treated animals showed no evidence of cancer recurrence. Thus, the developed therapeutic approach can be potentially employed intraoperatively to eradicate unresactable cancer cells. From the Clinical Editor: The complete clearance of microscopic residual tumor cells during excision surgery is important to improve survival of the patient. In this interesting paper, the authors developed a novel approach using targeted photodynamic therapy (PDT), combining a photosensitizer, phthalocyanine, and DJ-1 siRNA for the treatment of ovarian cancer. The data showed that this approach increased cancer cell killing and may pave way for future clinical studies.

Original languageEnglish (US)
Pages (from-to)1961-1970
Number of pages10
JournalNanomedicine: Nanotechnology, Biology, and Medicine
Volume11
Issue number8
DOIs
StatePublished - Nov 1 2015

Fingerprint

Photodynamic therapy
Oncology
Photochemotherapy
Ovarian Neoplasms
Genes
Cells
Photosensitizing Agents
Photosensitizers
Neoplasms
Small Interfering RNA
Tumors
Proteins
Therapeutics
Dendrimers
Surgery
Residual Neoplasm
Animals
Infrared radiation
Carcinoma
Recurrence

Keywords

  • DJ-1
  • Ovarian cancer
  • Photodynamic therapy
  • ROS
  • siRNA therapy

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Molecular Medicine
  • Biomedical Engineering
  • Materials Science(all)
  • Pharmaceutical Science

Cite this

ROS-induced nanotherapeutic approach for ovarian cancer treatment based on the combinatorial effect of photodynamic therapy and DJ-1 gene suppression. / Schumann, Canan; Taratula, Olena; Khalimonchuk, Oleh; Palmer, Amy L.; Cronk, Lauren M.; Jones, Carson V.; Escalante, Cesar A.; Taratula, Oleh.

In: Nanomedicine: Nanotechnology, Biology, and Medicine, Vol. 11, No. 8, 01.11.2015, p. 1961-1970.

Research output: Contribution to journalArticle

Schumann, Canan ; Taratula, Olena ; Khalimonchuk, Oleh ; Palmer, Amy L. ; Cronk, Lauren M. ; Jones, Carson V. ; Escalante, Cesar A. ; Taratula, Oleh. / ROS-induced nanotherapeutic approach for ovarian cancer treatment based on the combinatorial effect of photodynamic therapy and DJ-1 gene suppression. In: Nanomedicine: Nanotechnology, Biology, and Medicine. 2015 ; Vol. 11, No. 8. pp. 1961-1970.
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