Roles of TGF β and FGF signals in the lens: Tropomyosin regulation for posterior capsule opacity

Eri Kubo, Teppei Shibata, Dhirendra P. Singh, Hiroshi Sasaki

Research output: Contribution to journalReview article

3 Citations (Scopus)

Abstract

Transforming growth factor (TGF) β and fibroblast growth factor (FGF) 2 are related to the development of posterior capsule opacification (PCO) after lens extraction surgery and other processes of epithelial–mesenchymal transition (EMT). Oxidative stress seems to activate TGF β1 largely through reactive oxygen species (ROS) production, which in turn alters the transcription of several survival genes, including lens epithelium-cell derived growth factor (LEDGF). Higher ROS levels attenuate LEDGF function, leading to down-regulation of peroxiredoxin 6 (Prdx6). TGF β is regulated by ROS in Prdx6 knock-out lens epithelial cells (LECs) and induces the up-regulation of tropomyosins (Tpms) 1/2, and EMT of LECs. Mouse and rat PCO are accompanied by elevated expression of Tpm2. Further, the expression of Tpm1/2 is induced by TGF β2 in LECs. Importantly, we previously showed that TGF β2 and FGF2 play regulatory roles in LECs in a contrasting manner. An injury-induced EMT of a mouse lens as a PCO model was attenuated in the absence of Tpm2. In this review, we present findings regarding the roles of TGF β and FGF2 in the differential regulation of EMT in the lens. Tpms may be associated with TGF β2-and FGF2-related EMT and PCO development.

Original languageEnglish (US)
Article number3093
JournalInternational journal of molecular sciences
Volume19
Issue number10
DOIs
StatePublished - Oct 9 2018

Fingerprint

Tropomyosin
Fibroblast Growth Factors
Opacity
fibroblasts
Transforming Growth Factors
Fibroblasts
capsules
opacity
Lenses
Capsules
Capsule Opacification
lenses
Fibroblast Growth Factor 2
Peroxiredoxin VI
Epithelial Cells
Reactive Oxygen Species
epithelium
Intercellular Signaling Peptides and Proteins
Oxygen
mice

Keywords

  • Epithelial-mesenchymal transition
  • FGF
  • Lens epithelium-cell derived growth factor
  • Peroxiredoxin 6
  • Reactive oxygen species
  • TGF β
  • Tropomyosin

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Cite this

Roles of TGF β and FGF signals in the lens : Tropomyosin regulation for posterior capsule opacity. / Kubo, Eri; Shibata, Teppei; Singh, Dhirendra P.; Sasaki, Hiroshi.

In: International journal of molecular sciences, Vol. 19, No. 10, 3093, 09.10.2018.

Research output: Contribution to journalReview article

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abstract = "Transforming growth factor (TGF) β and fibroblast growth factor (FGF) 2 are related to the development of posterior capsule opacification (PCO) after lens extraction surgery and other processes of epithelial–mesenchymal transition (EMT). Oxidative stress seems to activate TGF β1 largely through reactive oxygen species (ROS) production, which in turn alters the transcription of several survival genes, including lens epithelium-cell derived growth factor (LEDGF). Higher ROS levels attenuate LEDGF function, leading to down-regulation of peroxiredoxin 6 (Prdx6). TGF β is regulated by ROS in Prdx6 knock-out lens epithelial cells (LECs) and induces the up-regulation of tropomyosins (Tpms) 1/2, and EMT of LECs. Mouse and rat PCO are accompanied by elevated expression of Tpm2. Further, the expression of Tpm1/2 is induced by TGF β2 in LECs. Importantly, we previously showed that TGF β2 and FGF2 play regulatory roles in LECs in a contrasting manner. An injury-induced EMT of a mouse lens as a PCO model was attenuated in the absence of Tpm2. In this review, we present findings regarding the roles of TGF β and FGF2 in the differential regulation of EMT in the lens. Tpms may be associated with TGF β2-and FGF2-related EMT and PCO development.",
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