Abstract
The Rho-related family of GTPases are pivotal regulators of morphogenetic processes in diverse eukaryotic organisms. In the filamentous fungi two related members of this family, Cdc42 and Rac1, perform particularly important roles in the establishment and maintenance of hyphal polarity. The activity of these GTPases is tightly controlled by two sets of regulators: guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs). Despite the importance of Cdc42 and Rac1 in polarized hyphal growth, the morphogenetic functions of their cognate GEFs and GAPs have not been widely characterized in filamentous fungi outside the Saccharomycotina. Here we present a functional analysis of the Aspergillus nidulans homologs of the yeast GEF Cdc24 and the yeast GAP Rga1. We show that Cdc24 is required for the establishment of hyphal polarity and localizes to hyphal tips. We also show that Rga1 is necessary for the suppression of branching in developing conidiophores. During asexual development Rga1 appears to act primarily via Cdc42 and in doing so serves as a critical determinant of conidiophore architecture. Our results provide new insight into the roles of Cdc42 during development in A. nidulans.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 543-555 |
| Number of pages | 13 |
| Journal | Mycologia |
| Volume | 108 |
| Issue number | 3 |
| DOIs | |
| State | Published - May 1 2016 |
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Keywords
- Aspergillus nidulans
- Cdc24
- Cdc42
- Conidiophore development
- Hyphal polarity
- Rga1
ASJC Scopus subject areas
- Ecology, Evolution, Behavior and Systematics
- Plant Science
- Cell Biology
- Genetics
- Molecular Biology
- Physiology
Cite this
Roles of Aspergillus nidulans Cdc42/Rho GTPase regulators in hyphal morphogenesis and development. / Si, Haoyu; Rittenour, William R.; Harris, Steven D.
In: Mycologia, Vol. 108, No. 3, 01.05.2016, p. 543-555.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Roles of Aspergillus nidulans Cdc42/Rho GTPase regulators in hyphal morphogenesis and development
AU - Si, Haoyu
AU - Rittenour, William R.
AU - Harris, Steven D.
PY - 2016/5/1
Y1 - 2016/5/1
N2 - The Rho-related family of GTPases are pivotal regulators of morphogenetic processes in diverse eukaryotic organisms. In the filamentous fungi two related members of this family, Cdc42 and Rac1, perform particularly important roles in the establishment and maintenance of hyphal polarity. The activity of these GTPases is tightly controlled by two sets of regulators: guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs). Despite the importance of Cdc42 and Rac1 in polarized hyphal growth, the morphogenetic functions of their cognate GEFs and GAPs have not been widely characterized in filamentous fungi outside the Saccharomycotina. Here we present a functional analysis of the Aspergillus nidulans homologs of the yeast GEF Cdc24 and the yeast GAP Rga1. We show that Cdc24 is required for the establishment of hyphal polarity and localizes to hyphal tips. We also show that Rga1 is necessary for the suppression of branching in developing conidiophores. During asexual development Rga1 appears to act primarily via Cdc42 and in doing so serves as a critical determinant of conidiophore architecture. Our results provide new insight into the roles of Cdc42 during development in A. nidulans.
AB - The Rho-related family of GTPases are pivotal regulators of morphogenetic processes in diverse eukaryotic organisms. In the filamentous fungi two related members of this family, Cdc42 and Rac1, perform particularly important roles in the establishment and maintenance of hyphal polarity. The activity of these GTPases is tightly controlled by two sets of regulators: guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs). Despite the importance of Cdc42 and Rac1 in polarized hyphal growth, the morphogenetic functions of their cognate GEFs and GAPs have not been widely characterized in filamentous fungi outside the Saccharomycotina. Here we present a functional analysis of the Aspergillus nidulans homologs of the yeast GEF Cdc24 and the yeast GAP Rga1. We show that Cdc24 is required for the establishment of hyphal polarity and localizes to hyphal tips. We also show that Rga1 is necessary for the suppression of branching in developing conidiophores. During asexual development Rga1 appears to act primarily via Cdc42 and in doing so serves as a critical determinant of conidiophore architecture. Our results provide new insight into the roles of Cdc42 during development in A. nidulans.
KW - Aspergillus nidulans
KW - Cdc24
KW - Cdc42
KW - Conidiophore development
KW - Hyphal polarity
KW - Rga1
UR - http://www.scopus.com/inward/record.url?scp=84975076384&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84975076384&partnerID=8YFLogxK
U2 - 10.3852/15-232
DO - 10.3852/15-232
M3 - Article
VL - 108
SP - 543
EP - 555
JO - Mycologia
JF - Mycologia
SN - 0027-5514
IS - 3
ER -