Roles of a solo LuxR in the biological control agent Lysobacter enzymogenes strain OH11

Guoliang Qian, Feifei Xu, Vittorio Venturi, Liangcheng Du, Fengquan Liu

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Lysobacter enzymogenes is a ubiquitous plant-associated and environmentally friendly bacterium emerging as a novel biological control agent of plant disease. This bacterium produces diverse antifungal factors, such as lytic enzymes and a secondary metabolite (heat-stable antifungal factor [HSAF]) having antifungal activity with a novel structure and mode of action. The regulatory mechanisms for biosynthesis of antifungal factors is largely unknown in L. enzymogenes. The solo LuxR proteins have been shown to be widespread, playing important roles in plant-associated bacteria. Here, we cloned and studied a solo LuxR protein, LesR, from L. enzymogenes strain OH11. Overexpression but not deletion of lesR significantly impaired HSAF biosynthesis levels and antimicrobial activities but did not show visible effect on production of major lytic enzymes. Overexpression of lesR also led to remarkably accelerated cell aggregation and induced production of a melanin-like pigment in L. enzymogenes; these two phenotypes are mediated by the diffusible factor cell-tocell signaling system of L. enzymogenes. The C-terminus helix-turn-helix domain was shown to be critical for several lesR-controlled functions. Overall, our study provides the first example of the roles and mechanisms of a solo LuxR protein in a plant-associated L. enzymogenes.

Original languageEnglish (US)
Pages (from-to)224-231
Number of pages8
JournalPhytopathology
Volume104
Issue number3
DOIs
StatePublished - Mar 1 2014

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Lysobacter enzymogenes
biological control agents
bacteria
biosynthesis
heat
melanin
plant proteins
plant diseases and disorders
enzymes
secondary metabolites
mechanism of action
proteins
anti-infective agents
pigments
cells
phenotype

Keywords

  • Quorum sensing

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Cite this

Roles of a solo LuxR in the biological control agent Lysobacter enzymogenes strain OH11. / Qian, Guoliang; Xu, Feifei; Venturi, Vittorio; Du, Liangcheng; Liu, Fengquan.

In: Phytopathology, Vol. 104, No. 3, 01.03.2014, p. 224-231.

Research output: Contribution to journalArticle

Qian, Guoliang ; Xu, Feifei ; Venturi, Vittorio ; Du, Liangcheng ; Liu, Fengquan. / Roles of a solo LuxR in the biological control agent Lysobacter enzymogenes strain OH11. In: Phytopathology. 2014 ; Vol. 104, No. 3. pp. 224-231.
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