Role of thromboxane A2 in the induction of apoptosis of immature thymocytes by lipopolysaccharide.

Paulo N. Rocha, Troy J Plumb, Lisa A. Robinson, Robert Spurney, David Pisetsky, Beverly H. Koller, Thomas M. Coffman

Research output: Contribution to journalArticle

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Abstract

Lipopolysaccharide (LPS) causes apoptotic deletion of CD4(+) CD8(+) thymocytes, a phenomenon that has been linked to immune dysfunction and poor survival during sepsis. Given the abundance of thromboxane-prostanoid (TP) receptors in CD4(+) CD8(+) thymocytes and in vitro evidence that thromboxane A(2) (TXA(2)) causes apoptosis of these cells, we tested whether enhanced generation of TXA(2) plays a role in LPS-induced thymocyte apoptosis. Mice injected with 50 micro LPS intraperitoneally displayed a marked increase in generation of TXA(2) and prostaglandin E(2) in the thymus as well as apoptotic deletion of CD4(+) CD8(+) thymocytes. Administration of indomethacin or rofecoxib inhibited prostanoid synthesis but did not affect thymocyte death. In contrast, thymocyte apoptosis in response to LPS was significantly attenuated in TP-deficient mice. These studies indicate that TXA(2) mediates a portion of apoptotic thymocyte death caused by LPS. The absence of an effect of global inhibition of prostanoid synthesis suggests a complex role for prostanoids in this model.

Original languageEnglish (US)
Pages (from-to)896-903
Number of pages8
JournalClinical and diagnostic laboratory immunology
Volume12
Issue number8
StatePublished - Jan 1 2005

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Thromboxane A2
Thromboxanes
Thymocytes
Lipopolysaccharides
Prostaglandins
Apoptosis
Thymus
Thromboxane Receptors
Prostaglandins E
Indomethacin
Thymus Gland
Sepsis

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Clinical Biochemistry
  • Microbiology (medical)

Cite this

Rocha, P. N., Plumb, T. J., Robinson, L. A., Spurney, R., Pisetsky, D., Koller, B. H., & Coffman, T. M. (2005). Role of thromboxane A2 in the induction of apoptosis of immature thymocytes by lipopolysaccharide. Clinical and diagnostic laboratory immunology, 12(8), 896-903.

Role of thromboxane A2 in the induction of apoptosis of immature thymocytes by lipopolysaccharide. / Rocha, Paulo N.; Plumb, Troy J; Robinson, Lisa A.; Spurney, Robert; Pisetsky, David; Koller, Beverly H.; Coffman, Thomas M.

In: Clinical and diagnostic laboratory immunology, Vol. 12, No. 8, 01.01.2005, p. 896-903.

Research output: Contribution to journalArticle

Rocha, PN, Plumb, TJ, Robinson, LA, Spurney, R, Pisetsky, D, Koller, BH & Coffman, TM 2005, 'Role of thromboxane A2 in the induction of apoptosis of immature thymocytes by lipopolysaccharide.', Clinical and diagnostic laboratory immunology, vol. 12, no. 8, pp. 896-903.
Rocha, Paulo N. ; Plumb, Troy J ; Robinson, Lisa A. ; Spurney, Robert ; Pisetsky, David ; Koller, Beverly H. ; Coffman, Thomas M. / Role of thromboxane A2 in the induction of apoptosis of immature thymocytes by lipopolysaccharide. In: Clinical and diagnostic laboratory immunology. 2005 ; Vol. 12, No. 8. pp. 896-903.
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