Role of the yeast DNA repair protein Nej1 in end processing during the repair of DNA double strand breaks by non-homologous end joining

Hui Yang, Yoshihiro Matsumoto, Kelly M. Trujillo, Susan P. Lees-Miller, Mary Ann Osley, Alan E. Tomkinson

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

DNA double strand breaks (DSB)s often require end processing prior to joining during their repair by non-homologous end joining (NHEJ). Although the yeast proteins, Pol4, a Pol X family DNA polymerase, and Rad27, a nuclease, participate in the end processing reactions of NHEJ, the mechanisms underlying the recruitment of these factors to DSBs are not known. Here we demonstrate that Nej1, a NHEJ factor that interacts with and modulates the activity of the NHEJ DNA ligase complex (Dnl4/Lif1), physically and functionally interacts with both Pol4 and Rad27. Notably, Nej1 and Dnl4/Lif1, which also interacts with both Pol4 and Rad27, independently recruit the end processing factors to in vivo DSBs via mechanisms that are additive rather than redundant. As was observed with Dnl4/Lif1, the activities of both Pol4 and Rad27 were enhanced by the interaction with Nej1. Furthermore, Nej1 increased the joining of incompatible DNA ends in reconstituted reactions containing Pol4, Rad27 and Dnl4/Lif1, indicating that the stimulatory activities of Nej1 and Dnl4/Lif1 are also additive. Together our results reveal novel roles for Nej1 in the recruitment of Pol4 and Rad27 to in vivo DSBs and the coordination of the end processing and ligation reactions of NHEJ.

Original languageEnglish (US)
Pages (from-to)1-10
Number of pages10
JournalDNA Repair
Volume31
DOIs
StatePublished - Jul 1 2015

Fingerprint

Double-Stranded DNA Breaks
Joining
DNA Repair
Yeast
Repair
Yeasts
DNA
Processing
DNA Ligases
Proteins
Fungal Proteins
Ligation
DNA-Directed DNA Polymerase
1,2-di-(4-sulfamidophenyl)-4-butylpyrazolidine-3,5-dione

Keywords

  • DNA damage
  • DNA double-strand break
  • DNA ligase
  • DNA nuclease
  • DNA polymerase
  • Genomic instability
  • Protein-protein interaction

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Role of the yeast DNA repair protein Nej1 in end processing during the repair of DNA double strand breaks by non-homologous end joining. / Yang, Hui; Matsumoto, Yoshihiro; Trujillo, Kelly M.; Lees-Miller, Susan P.; Osley, Mary Ann; Tomkinson, Alan E.

In: DNA Repair, Vol. 31, 01.07.2015, p. 1-10.

Research output: Contribution to journalArticle

Yang, Hui ; Matsumoto, Yoshihiro ; Trujillo, Kelly M. ; Lees-Miller, Susan P. ; Osley, Mary Ann ; Tomkinson, Alan E. / Role of the yeast DNA repair protein Nej1 in end processing during the repair of DNA double strand breaks by non-homologous end joining. In: DNA Repair. 2015 ; Vol. 31. pp. 1-10.
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