Role of the Escherichia coli FadR regulator in stasis survival and growth phase-dependent expression of the uspA, fad, and fab genes

Anne Farewell, Alfredo A. Diez, Concetta C. DiRusso, Thomas Nyström

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Abstract

The increased expression of the uspA gene of Escherichia coli is an essential part of the cell's response to growth arrest. We demonstrate that stationary-phase activation of the uspA promoter is in part dependent on growth phase-dependent inactivation or repression of the FadR regulator. Transcription of uspA is derepressed during exponential growth in fadR null mutants or by including the fatty acid oleate in the growth medium of FadR+ cells. The results of DNA footprinting analysis show that FadR binds downstream of the uspA promoter in the noncoding region. Thus, uspA is a member of the fadR regulon. All the fad-lacZ fusions examined (fadBA, fadL, and fadD) are increasingly expressed in stationary phase with kinetics similar to that of the increased expression of uspA. In contrast, β- galactosidase levels decrease during stationary phase in a fabA-lacZ lysogen, consistent with the role of FadR as an activator of fabA. The growth phase- dependent increased and decreased transcription of fad genes and fabA, respectively, is dependent on the status of the fadR gene. Cells carrying a mutation in the FadR gene (fadRS219N) that makes it nonderepressible exhibit a weak stationary-phase induction of uspA and fad genes. In addition, cells carrying fadRS219N survive long-term stasis poorly, indicating that FadR- dependent alterations in fatty acid metabolism are an integral and important part of the adaptation to stationary phase.

Original languageEnglish (US)
Pages (from-to)6443-6450
Number of pages8
JournalJournal of bacteriology
Volume178
Issue number22
DOIs
StatePublished - Jan 1 1996

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Escherichia coli
Growth
Genes
DNA Footprinting
Fatty Acids
Galactosidases
Regulon
Oleic Acid
Gene Expression
Mutation

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Role of the Escherichia coli FadR regulator in stasis survival and growth phase-dependent expression of the uspA, fad, and fab genes. / Farewell, Anne; Diez, Alfredo A.; DiRusso, Concetta C.; Nyström, Thomas.

In: Journal of bacteriology, Vol. 178, No. 22, 01.01.1996, p. 6443-6450.

Research output: Contribution to journalArticle

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