Role of prostaglandin H2-thromboxane A2 in responses of cerebral arterioles during chronic hypertension

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Abstract

The goal of this study was to determine the role of prostaglandin H2- thromboxane A2 (PGH2-TxA2) in altered responses of cerebral arterioles during chronic hypertension. Diameter of pial arterioles was measured during suffusion with ADP, acetylcholine, and nitroglycerin using intravital microscopy in Wistar-Kyoto (WKY) normotensive rats and spontaneously hypertensive rats (SHR) (8-10 mo old). ADP (100 μM) increased pial arteriolar diameter by 21 ± 3% (means ± SE) in WKY and only by 7 ± 3% in SHR. Acetylcholine (10 μM) increased diameter 10 ± 2% in WKY and, in contrast, reduced diameter 7 ± 3% in SHR. Nitroglycerin produced similar vasodilatation in WKY and SHR. We then examined whether impaired dilatation of cerebral arterioles in SHR to ADP and acetylcholine may be related to activation of the PGH2-TxA2 receptor. SQ 29548, a specific PGH2-TxA2 receptor antagonist, restored vasodilatation in response to ADP in SHR toward that observed in WKY and reversed vasoconstriction to vasodilatation in response to acetylcholine in SHR. SQ 29548 did not alter responses in WKY. Thus these findings suggest that impaired responses of cerebral arterioles to ADP and acetylcholine during chronic hypertension may be related to the activation of the PGH2-TxA2 receptor.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume262
Issue number2 31-2
StatePublished - 1992

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Prostaglandin H2
Thromboxane A2
Arterioles
Inbred SHR Rats
Hypertension
Adenosine Diphosphate
Acetylcholine
Prostaglandin H2 Receptors Thromboxane A2
Vasodilation
Nitroglycerin
Inbred WKY Rats
Vasoconstriction
Dilatation

Keywords

  • acetylcholine
  • adenosine 5'-diphosphate
  • endothelium-derived relaxing factor
  • nitroglycerin
  • spontaneously hypertensive rats
  • SQ 29548
  • Wistar-Kyoto rats

ASJC Scopus subject areas

  • Physiology
  • Medicine(all)

Cite this

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title = "Role of prostaglandin H2-thromboxane A2 in responses of cerebral arterioles during chronic hypertension",
abstract = "The goal of this study was to determine the role of prostaglandin H2- thromboxane A2 (PGH2-TxA2) in altered responses of cerebral arterioles during chronic hypertension. Diameter of pial arterioles was measured during suffusion with ADP, acetylcholine, and nitroglycerin using intravital microscopy in Wistar-Kyoto (WKY) normotensive rats and spontaneously hypertensive rats (SHR) (8-10 mo old). ADP (100 μM) increased pial arteriolar diameter by 21 ± 3{\%} (means ± SE) in WKY and only by 7 ± 3{\%} in SHR. Acetylcholine (10 μM) increased diameter 10 ± 2{\%} in WKY and, in contrast, reduced diameter 7 ± 3{\%} in SHR. Nitroglycerin produced similar vasodilatation in WKY and SHR. We then examined whether impaired dilatation of cerebral arterioles in SHR to ADP and acetylcholine may be related to activation of the PGH2-TxA2 receptor. SQ 29548, a specific PGH2-TxA2 receptor antagonist, restored vasodilatation in response to ADP in SHR toward that observed in WKY and reversed vasoconstriction to vasodilatation in response to acetylcholine in SHR. SQ 29548 did not alter responses in WKY. Thus these findings suggest that impaired responses of cerebral arterioles to ADP and acetylcholine during chronic hypertension may be related to the activation of the PGH2-TxA2 receptor.",
keywords = "acetylcholine, adenosine 5'-diphosphate, endothelium-derived relaxing factor, nitroglycerin, spontaneously hypertensive rats, SQ 29548, Wistar-Kyoto rats",
author = "William Mayhan",
year = "1992",
language = "English (US)",
volume = "262",
journal = "American Journal of Physiology - Renal Physiology",
issn = "0363-6127",
publisher = "American Physiological Society",
number = "2 31-2",

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TY - JOUR

T1 - Role of prostaglandin H2-thromboxane A2 in responses of cerebral arterioles during chronic hypertension

AU - Mayhan, William

PY - 1992

Y1 - 1992

N2 - The goal of this study was to determine the role of prostaglandin H2- thromboxane A2 (PGH2-TxA2) in altered responses of cerebral arterioles during chronic hypertension. Diameter of pial arterioles was measured during suffusion with ADP, acetylcholine, and nitroglycerin using intravital microscopy in Wistar-Kyoto (WKY) normotensive rats and spontaneously hypertensive rats (SHR) (8-10 mo old). ADP (100 μM) increased pial arteriolar diameter by 21 ± 3% (means ± SE) in WKY and only by 7 ± 3% in SHR. Acetylcholine (10 μM) increased diameter 10 ± 2% in WKY and, in contrast, reduced diameter 7 ± 3% in SHR. Nitroglycerin produced similar vasodilatation in WKY and SHR. We then examined whether impaired dilatation of cerebral arterioles in SHR to ADP and acetylcholine may be related to activation of the PGH2-TxA2 receptor. SQ 29548, a specific PGH2-TxA2 receptor antagonist, restored vasodilatation in response to ADP in SHR toward that observed in WKY and reversed vasoconstriction to vasodilatation in response to acetylcholine in SHR. SQ 29548 did not alter responses in WKY. Thus these findings suggest that impaired responses of cerebral arterioles to ADP and acetylcholine during chronic hypertension may be related to the activation of the PGH2-TxA2 receptor.

AB - The goal of this study was to determine the role of prostaglandin H2- thromboxane A2 (PGH2-TxA2) in altered responses of cerebral arterioles during chronic hypertension. Diameter of pial arterioles was measured during suffusion with ADP, acetylcholine, and nitroglycerin using intravital microscopy in Wistar-Kyoto (WKY) normotensive rats and spontaneously hypertensive rats (SHR) (8-10 mo old). ADP (100 μM) increased pial arteriolar diameter by 21 ± 3% (means ± SE) in WKY and only by 7 ± 3% in SHR. Acetylcholine (10 μM) increased diameter 10 ± 2% in WKY and, in contrast, reduced diameter 7 ± 3% in SHR. Nitroglycerin produced similar vasodilatation in WKY and SHR. We then examined whether impaired dilatation of cerebral arterioles in SHR to ADP and acetylcholine may be related to activation of the PGH2-TxA2 receptor. SQ 29548, a specific PGH2-TxA2 receptor antagonist, restored vasodilatation in response to ADP in SHR toward that observed in WKY and reversed vasoconstriction to vasodilatation in response to acetylcholine in SHR. SQ 29548 did not alter responses in WKY. Thus these findings suggest that impaired responses of cerebral arterioles to ADP and acetylcholine during chronic hypertension may be related to the activation of the PGH2-TxA2 receptor.

KW - acetylcholine

KW - adenosine 5'-diphosphate

KW - endothelium-derived relaxing factor

KW - nitroglycerin

KW - spontaneously hypertensive rats

KW - SQ 29548

KW - Wistar-Kyoto rats

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VL - 262

JO - American Journal of Physiology - Renal Physiology

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